Electronic Theses and Dissertation

Newcastle disease merupakan salah satu penyakit yang sangat penting pada unggas karena sangat menular dan menyebabkan kerugian ekonomi yang besar. Pencegahan Newcastle disease terus dilakukan, namun penyakit ini tetap saja terus terjadi. Penelitian ini bertujuan untuk mengetahui gambaran histopatologi dan derajat sebaran lesi histopatologi proventrikulus ayam buras yang mati akibat Newcastle disease pada kasus lapang. Penelitian ini menggunakan metode observasional kualitatif, dengan sampel berupa 5 blok parafin yang berisi jaringan proventrikulus ayam buras positif terinfeksi virus Newcastle disease berdasarkan uji imunohistokimia. Sampel dipreparasi untuk dilakukan pewarnaan hematoksilin dan eosin. Pengamatan dilakukan pada 5 lapang pandang dengan perbesaran 100x terhadap parameter lesi berupa hiperemi, hemoragi, infiltrasi sel-sel radang dan deskuamasi sel epitel. Derajat sebaran lesi ditentukan berdasarkan kategori fokal, multifokal dan difus. Hasil pengamatan histopatologi pada semua sampel proventrikulus ditemukan adanya hiperemi, hemoragi, infiltrasi sel-sel radang dan deskuamasi sel epitel dengan derajat sebaran lesi hiperemi, hemoragi dan infiltrasi sel-sel radang tersebar multifokal dan derajat sebaran lesi deskuamasi sel epitel tersebar secara multifokal hingga difus. Gambaran histopatologi proventrikulus ayam buras yang mati akibat penyakit Newcastle disease pada kasus lapang terlihat adanya lesi hiperemi, hemoragi, infiltrasi sel-sel radang dan deskuamasi sel epitel dengan derajat sebaran lesi tersebar multifokal hingga difus.

Newcastle disease is one of the most important diseases in poultry because it is highly contagious and causes great economic losses. Prevention of Newcastle disease continues to be carried out, but this disease continues to occur. This study aims to determine the histopathological findings and the degree of distribution of histopathological lesions in the proventriculus domestic chickens that died due to Newcastle disease in field cases. This study used a qualitative observational method, with samples in the form of 5 paraffin blocks containing proventriculus tissue of domestic chickens positive Newcastle disease virus based on immunohistochemical tests. Samples were prepared for hematoxylin and eosin staining. Observations were made in 5 fields of view with a magnification of 100x for lesion paramater including hyperemia, hemorrhagea, infiltration of inflamatory cells and desquamation of epithelial cells. The degree of lesion distribution was determined based on the categories of focal, multifocal and diffuse. The results of histopathological observations on all proventriculus samples found hyperemia, hemorrhage, infiltration of inflammatory cells and desquamation of epithelial cells with the degree of distribution on hyperemia, hemorrhagea and infiltration of inflammatory cells spread multifocally and the degree of distribution on desquamation of epithelial cells lesions spread multifocally and diffusely. The histopathological findings of the proventriculus domestic chickens that died due to Newcastle disease from field cases showed hyperemia, hemorrhagea, infiltration of inflammatory cells and desquamation of epithelial cells with the degree of distribution spread multifocally to diffusely.