Electronic Theses and Dissertation

Ekstrak hipofisa sapi (EHS) mengandung hormon gonadotropin seperti FSH dan LH yang berperan dalam regulasi fungsi gonad. Penelitian ini bertujuan mengetahui peningkatan morfometri dan histomorfometri testis tikus setelah pemberian EHS. Dalam penelitian ini digunakan 21 ekor tikus Wistar jantan dengan bobot badan 180-220 g. Seluruh tikus diadaptasikan selama dua minggu. Tikus dibagi ke dalam tujuh kelompok perlakuan (n=3), yakni 1 kelompok sebagai kontrol (P0) dan 6 kelompok (P1-P6) mendapat perlakuan dengan EHS. Pada P0, tikus diinjeksi dengan NaCl 0,9% selama 1 minggu sedangkan P1-P6 tikus yang diinjeksi dengan 3 IU EHS/tikus selama 1 minggu (P1), 2 minggu (P2), 3 minggu (P3), 4 minggu (P4), 5 minggu (P5), dan 6 minggu (P6). Masing-masing kelompok diinjeksi sebanyak tiga kali dalam seminggu. Setelah perlakuan selesai, tikus dieutanasi dengan 40 mg/kg zoletil dan testis dikoleksi untuk pengamatan morfologi, pengukuran morfometri, dan pembuatan preparat histologis. Pengukuran morfometri pada testis meliputi panjang, lebar, lingkar, volume dan berat. Pengukuran histomorfometri testis meliputi diameter tubulus seminiferus dan ketebalan dari lapisan sel epitel germinal yang diukur mulai dari membran basal sampai adluminal tubulus seminiferus testis. Data yang diperoleh pada penelitian ini dianalisis menggunakan analisis varian satu arah dan dilanjutkan dengan uji Duncan. Seluruh parameter morfologi testis tikus pada kelompok P5 memiliki perbedaan yang signifikan dengan P0 (P

Bovine pituitary extract (BPE) contains gonadotropin hormones such as FSH and LH which play a role in regulating gonadal function. This study aims to determine the increase in morphometry and histomorphometry of rat testis after BPE administration. In this study, 21 male Wistar rats with a body weight of 180-220 g were used. All rats were adapted for two weeks. The rats were divided into seven treatment groups (n = 3), namely 1 group as a control (P0) and 6 groups (P1-P6) treated with BPE. At P0, rats were injected with 0.9% NaCl for 1 week while P1-P6 rats were injected with 3 IU BPE/rat for 1 week (P1), 2 weeks (P2), 3 weeks (P3), 4 weeks (P4), 5 weeks (P5), and 6 weeks (P6). Each group was injected three times a week. After the treatment was completed, the rats were euthanized with 40 mg/kg zoletil and the testis were collected for morphological observation, morphometric measurements, and histological preparations. Morphometric measurements of the testes included length, width, circumference, volume, and weight. Histomorphometric measurements of the testes included the diameter of the seminiferous tubules and the thickness of the germinal epithelial cell layer measured from the basal membrane to the adluminal of the seminiferous tubules of the testis. The data obtained in this study were analyzed using one-way analysis of variance and continued with the Duncan test. All morphological parameters of the rat testes in the P5 group had significant differences with P0 (P