Electronic Theses and Dissertation

Antibiotik sering digunakan sebagai pengobatan terhadap infeksi mikroorganisme. Colistin adalah salah satu antibiotik untuk mengatasi colibasilosis. Namun, antibiotik ini memiliki spektrum sempit untuk melawan bakteri Gram negatif. Penelitian ini bertujuan untuk mengisolasi Escherichia coli patogen dari sampel usus ayam dan menghitung persentase resisten colistin pada E. coli patogen isolat asal ayam broiler. Penelitian ini menggunakan metode observasional lapangan dengan pendekatan Cross sectional study. Isolasi bakteri dikerjakan sesuai dengan metode Carter (1987). Isolasi dan identifikasi Escherichia coli menggunakan media Eosin Methylen Blue Agar (EMBA), hasil pewarnaan Gram bakteri termasuk kelompok Gram negatif yang berwarna pink dan berbentuk cocobacilus, penanaman pada media Sorbitol MacConkey Agar (SMAC) dan uji resistensi antibiotik menggunakan metode Kirby-Bauer. Hasil penelitian menunjukkan bahwa dari 15 sampel yang diperiksa terdapat 13 sampel yang menunjukkan hasil positif Escherichia coli. 13 isolat E. coli dilanjutkan penanaman pada media Sorbitol MacConkey Agar menunjukan hasil positif Escherichia coli patogen. Hasil uji resisten terhadap antibiotik menunjukkan bahwa sebesar 86,67% E. coli patogen telah resisten terhadap colistin. Dari penelitian ini dapat disimpulkan bahwa colistin telah resisten terhadap Escherichia coli patogen isolat asal ayam broiler.

Antibiotics were often used as a treatment for microorganism infections. Colistin was one of the antibiotics used to treat colibacillosis. However, this antibiotic had a narrow spectrum against Gram-negative bacteria. This study aimed to isolate pathogenic Escherichia coli from chicken intestinal samples and to calculate the percentage of colistin resistance in pathogenic E. coli isolated from broiler chicken. This research used a field observational method with a Cross-sectional study method. Bacterial isolation was carried out according to Carter's (1987) method. Isolation and identification of Escherichia coli used Eosin Methylene Blue Agar (EMBA) media. The Gram staining of the bacteria showed they belonged to the Gram-negative group, appearing pink and coccobacillus-shaped, then followed by planting on Sorbitol MacConkey Agar (SMAC), and antibiotic resistance testing was conducted using the Kirby-Bauer method. The results of the research indicated that out of 15 samples examined, there were 13 samples showed positive results for Escherichia coli. Then 13 E. coli isolates were continued to be planted on Sorbitol MacConkey Agar media showed positive results for pathogenic Escherichia coli. The antibiotic resistance test results showed that 86,67% of pathogenic E. coli had become resistant to colistin. From this research, it can concluded that colistin had become resistant to pathogenic Escherichia coli isolates from broiler chickens.