Electronic Theses and Dissertation

Penyakit Newcastle disease (ND) merupakan penyakit menular tinggi pada unggas yang disebabkan oleh virus Newcastle disease (VND). Kemampuan VND untuk menginfeksi berbagai spesies burung menunjukkan sebaran virus yang luas dan berdampak pada populasi burung liar. Penelitian ini bertujuan untuk mengetahui ada atau tidak kuntul kerbau (Bubulcus ibis) yang positif terinfeksi VND di Hutan Mangrove Kecamatan Baitussalam Kabupaten Aceh Besar. Penelitian ini menggunakan metode observasional dengan sampel penelitian berupa 33 ekor kadaver kuntul kerbau (Bubulcus ibis) yang dikoleksi organnya berupa paru, otak, proventrikulus, dan duodenum. Organ-organ tersebut diproses menjadi sediaan paraffin blok dan dilakukan pewarnaan Imunohistokimia (IHK) dengan metode ABC pada manual kit mouse and rabbit specific HRP/DAB (ABC) detection IHC kit. Hasil pewarnaan IHK diamati dan dianalisis secara deskriptif untuk mengidentifikasi kuntul kerbau (Bubulcus ibis) yang positif terinfeksi VND. Hasil positif pewarnaan imunohistokimia terlihat di sitoplasma sel yang terwarnai coklat pada otak, paru, proventrikulus, dan duodenum. Hasil penelitian terdapat 8 dari 33 ekor (24,2%) kadaver kuntul kerbau (Bubulcus ibis) positif terinfeksi VND.

Kata kunci: Imunohistokimia, kuntul kerbau, Newcastle disease.

Newcastle disease (ND) is a highly contagious disease in poultry caused by the Newcastle disease virus (NDV). The ability of NDV to infect various bird species indicates a wide distribution of the virus and its impact on wild bird populations. This study aims to determine whether there are cattle egrets (Bubulcus ibis) that test positive for NDV infection in the Mangrove Forest of Baitussalam District, Aceh Besar Regency. The research employed an observational method with a sample consisting of 33 cattle egret cadavers (Bubulcus ibis), from which organs such as the lungs, brain, proventriculus, and duodenum were collected. These organs were processed into paraffin blocks and subjected to immunohistochemical (IHC) staining using the ABC method with a manual kit for mouse and rabbit specific HRP/DAB (ABC) detection IHC kit. The results of the IHC staining were observed and analyzed descriptively to identify cattle egrets (Bubulcus ibis) that tested positive for NDV. Positive IHC staining results were observed in the cytoplasm of cells stained brown in the brain, lungs, proventriculus, and duodenum. The study found that 8 out of 33 (24.2%) cattle egret cadavers (Bubulcus ibis) tested positive for NDV infection. Keywords: Cattle egret, immunohistochemistry, Newcastle disease.