Electronic Theses and Dissertation

Penelitian ini bertujuan untuk mengembangkan film aktif berbasis pati aren dan kitosan dengan penambahan ekstrak bunga telang (Clitoria ternatea) untuk mengevaluasi aktivitas antioksidan, antimikroba, responsivitas pH, dan aplikasinya pada udang segar sebagai indikator kesegaran. Film aktif dibuat dalam variasi konsentrasi bunga telang (5%, 10%, dan 15%) dan diuji menggunakan metode DPPH untuk aktivitas antioksidan, uji zona hambat terhadap Staphylococcus aureus dan Escherichia coli, serta perubahan warna pada buffer pH yang berbeda. Selanjutnya, aplikasi film aktif pada udang segar dilakukan untuk mengamati perubahan warna terkait kesegaran produk selama penyimpanan. Hasil penelitian menunjukkan bahwa penambahan bunga telang secara signifikan meningkatkan aktivitas antioksidan film. Film kontrol (CH/SPS) hanya memiliki aktivitas antioksidan sebesar 11,70%, sedangkan film dengan ekstrak bunga telang BP05, BP10, dan BP15 masing-masing mencapai 58,32%, 60,91%, dan 61,93%. Hasil uji antimikroba, film BP10 menghasilkan zona hambat terbesar terhadap Staphylococcus aureus (10,18 ± 0,28 mm), sementara BP15 memberikan zona hambat terbesar terhadap Escherichia coli (18,35 ± 2,06 mm). Film aktif menunjukkan responsivitas pH yang baik dengan perubahan warna yang signifikan mulai dari ungu (asam), biru (netral), hingga hijau (basa). Aplikasi pada udang segar, film aktif berhasil mendeteksi perubahan pH yang terjadi secara bertahap selama penyimpanan. Selain itu, pada kondisi dingin pH udang tetap di bawah 7,7 selama 4 hari, dengan perubahan warna film dari ungu hingga kehijauan sesuai pH. Film aktif berbasis pati aren, kitosan, dan bunga telang memiliki potensi sebagai bahan kemasan pangan yang fungsional dikarenakan memiliki aktivitas antioksidan dan antimikroba yang baik, sifat responsivitas pH film memungkinkan deteksi visual kesegaran produk secara praktis, sehingga mendukung keamanan dan kualitas pangan.

This study aims to develop an active film based on palm starch and chitosan with the addition of telang flower extract (Clitoria ternatea) to evaluate its antioxidant, antimicrobial, pH responsiveness, and application in fresh shrimp as an indicator of freshness. The active film was created in variations in the concentration of telang flowers (5%, 10%, and 15%) and tested using the DPPH method for antioxidant activity, inhibition zone tests against Staphylococcus aureus and Escherichia coli, as well as discoloration at different pH buffers. Furthermore, the application of an active film on fresh shrimp is carried out to observe color changes related to the freshness of the product during storage. The results showed that the addition of telang flowers significantly increased the antioxidant activity of the film. The control film (CH/SPS) only had antioxidant activity of 11.70%, while the film with telang flower extract BP05, BP10, and BP15 reached 58.32%, 60.91%, and 61.93%, respectively. The results of the antimicrobial test, the BP10 film produced the largest inhibition zone against Staphylococcus aureus (10.18 ± 0.28 mm), while BP15 provided the largest inhibition zone against Escherichia coli (18.35 ± 2.06 mm). The active film exhibits good pH responsiveness with significant color changes ranging from purple (acidic), blue (neutral), to green (alkaline). Application to fresh shrimp, the active film successfully detects the pH changes that occur gradually during storage. In addition, in cold conditions the pH of the shrimp remained below 7.7 for 4 days, with the color of the film changing from purple to greenish according to the pH. Active films based on palm starch, chitosan, and telang flower have the potential as functional food packaging materials because they have good antioxidant and antimicrobial activity, the pH responsiveness of the film allows for visual detection of product freshness practically, thus supporting food safety and quality.