Electronic Theses and Dissertation

Pemeliharaan gajah sumatera pada lembaga konservasi haruslah memenuhi
prinsip kesejahteraan hewan, salah satunya bebas dari rasa stres. Kortisol adalah hormon
yang disekresikan sebagai respon terhadap stres, sehingga hormon ini dapat dijadikan
sebagai indikator stres. Perbedaan konsentrasi kortisol di tubuh dipengaruhi banyak hal
diantaranya jenis kelamin dan umur. Sampel feses dapat digunakan untuk mengukur
metabolit kortisol dengan metode invasif. Pengukuran metabolit kortisol dapat dilakukan
dengan kit komersial, namun perlu dilakukan uji validasi untuk memperoleh hasil yang
akurat. Tujuan penelitian ini adalah mengetahui validitas dari kit ELISA kortisol EIA-1887
(DRG Instruments GmbH, Germany) dalam mengukur konsentrasi metabolit kortisol dan
mengetahui konsentrasi metabolit kortisol pada jenis kelamin dan kelompok umur berbeda
pada gajah sumatera. Validasi kit ELISA meliputi validasi analitik (uji parallelism, uji
akurasi, dan uji presisi) dan validasi biologis. Pengukuran konsentrasi metabolit kortisol
dilakukan dengan mengelompokkan gajah berdasarkan jenis kelamin dan kategori umur.
Data uji parallelism dianalisis dengan uji korelasi, hasil uji akurasi dianalisis dengan
menghitung persentase recovery (% recovery) dan uji presisi dianalisis dengan menghitung
persentase coefficient variation (%CV) intra dan inter assay. Data konsentrasi metabolit
kortisol dengan jenis kelamin berbeda dianalisis dengan Mann-Whitney U test, dan
konsentrasi metabolit kortisol pada kategori umur berbeda dianalisis dengan KruskalWallis H test dan apabila ada perbedaan antar kelompok dilanjutkan dengan Mann-Whitney
U test. Hasil penelitian uji parallelism menunjukkan kurva yang sejajar antara kurva
standar dan sampel dengan r=0,996 dan p=0,004. Hasil Uji akurasi adalah 100,24% dan
hasil Uji presisi %CV intra assay adalah 2,74% untuk low quality control dan 3,89 % untuk
high quality control dan %CV inter assay adalah 3,08% untuk low quality control dan
5,75% untuk high quality control. Hasil uji validasi menunjukkan adanya variasi
konsentrasi metabolit kortisol pada gajah bunting trimester akhir dan gajah setelah
melahirkan (postpartus). Konsentrasi metabolit kortisol berbeda sangat signifikan (P

The care of Sumatran elephants in conservation institutions must meet the principles of animal welfare, one of which is free from stress. Cortisol is a hormone secreted in response to stress, so this hormone can be used as an indicator of stress. The difference in cortisol concentration in the body is influenced by many things including gender and age. Stool samples can be used to measure cortisol metabolites with invasive methods. Measurement of cortisol metabolites can be done with commercial kits, but validation tests need to be done to obtain accurate results. The purpose of this study was to determine the validity of the EIA-1887 cortisol ELISA kit (DRG Instruments GmbH, Germany) in measuring the concentration of cortisol metabolites and to determine the concentration of cortisol metabolites in different gender and age groups in Sumatran elephants. Validation of ELISA kits includes analytical validation (parallelism test, accuracy test, and precession test) and biological validation. Measurement of cortisol metabolite concentrations was carried out by grouping elephants based on sex and age categories. The parallelism test data were analyzed by correlation test, the accuracy test results were analyzed by calculating the percentage of recovery (% recovery) and the precision test was analyzed by calculating the percentage coefficient variation (%CV) intra and inter assay. Data on cortisol metabolite concentrations with different gender were analyzed by Mann-Whitney U test, and cortisol metabolite concentrations in different age categories were analyzed by Kruskal-Wallis H test and if there was a difference between groups continued with Mann-Whitney U test. The results of the parallelism test showed a parallel curve between the standard curve and the sample with r=0.996 and p=0.004. The accuracy test results were 100.24% and the %CV intra assay precision test results were 2.74% for low quality control and 3.89% for high quality control and %CV inter assay was 3.08% for low quality control and 5.75% for high quality control. The validation test results showed variations in cortisol metabolite concentrations in late trimester pregnant elephants and elephants after giving birth (postpartus). The concentration of cortisol metabolites was significantly different (P