Electronic Theses and Dissertation

Serai dapur (Cymbopogon citratus (DC.) Stapf) merupakan tanaman obat yang telah dipercaya sejak dahulu sebagai pengobatan tradisional dengan potensi efek yang menguntungkan bagi kesehatan. Kandungan senyawa metabolit sekunder dalam serai dapur telah dilaporkan berpotensi sebagai antiinflamasi. Penelitian ini bertujuan untuk menentukan aktivitas antiinflamasi dari ekstrak etanol 80% aerial parts C. citratus secara in vitro dengan metode denaturasi protein. Metode pengujian yang dilakukan meliputi skirining fitokimia, karakterisasi ekstrak, dan pengujian antiinflmasi dengan metode denaturasi protein yang diinduksi oleh faktor pemanasan terhadap protein Bovine Serum Albumin (BSA). Aktivitas antiinflamasi dilihat dari persentase inhibisi denaturasi protein yang menunjukkan kemampuan ekstrak dalam menghambat denaturasi protein. Hasil penelitian menunjukkan bahwa ekstrak etanol 80% C. citratus positif mengandung senyawa metabolit sekunder alkaloid, flavonoid, tanin, saponin dan terpenoid. Karakteristik ekstrak etanol 80% C. citratus memiliki kadar air sebesar 2,67%, kadar sari larut air 53%, kadar sari larut etanol 82,83% dan kadar abu total 4,17%. Hasil persentase inhibisi denaturasi protein ekstrak etanol 80% C. citratus pada konsentrasi 125, 250, 500 dan 1000 ppm berturut-turut adalah sebesar 52,744%; 65,670%; 69,353%; dan 79,221%. Nilai IC50 ekstrak etanol 80% C. citratus dan Na diklofenak berturut-turut adalah 87,461 ppm dan 34,828 ppm. Ekstrak dengan konsentrasi 1000 ppm menunjukkan tidak adanya perbedaan secara bermakna dengan Na diklofenak pada konsentrasi 500 ppm (P ≥0.05). Berdasarkan hasil penelitian ini dapat disimpulkan bahwa tanaman C. citratus bagian aerial parts memiliki aktivitas antiinflamasi dan berpotensi dikembangkan lebih lanjut sebagai kandidat obat antiinflamasi.


Kata kunci: antiinflamasi, ekstrak etanol 80%, serai dapur, Cymbopogon citratus, denaturasi protein, Bovine Serum Album (BSA)

Lemongrass (Cymbopogon citratus (DC.) Stapf) is a medicinal plant that has long been trusted as a traditional medicine with potential beneficial effects on health. The content of secondary metabolite compounds in kitchen lemongrass is reported to have anti-inflammatory potential. This study aims to determine the anti-inflammatory activity of 80% ethanol extract of C. citratus aerial parts in vitro using the protein denaturation method. The test methods used include phytochemical screening, extract characterization, and anti-inflammatory testing using a protein denaturation method induced by a heating factor on Bovine Serum Albumin (BSA) protein. Anti-inflammatory activity can be seen from the percentage of protein denaturation inhibition which shows the extract's ability to inhibit protein denaturation. The results showed that the 80% ethanol extract of C. citratus positively contained secondary metabolite compounds of alkaloids, flavonoids, tannins, saponins and terpenoids. Characteristics of the 80% ethanol extract of C. citratus is that it has a water content of 2.67%, a water soluble extract content of 53%, an ethanol soluble extract content of 82.83% and a total ash content of 4.17%. The percentage of inhibition of protein denaturation of 80% ethanol extract of C. citratus at concentrations of 125, 250, 500 and 1000 ppm respectively was 52.744%; 65.670%; 69.353%; and 79.221%. The IC50 values of 80% ethanol extract of C. citratus and Na diclofenac were 87,461 ppm and 34,828 ppm respectively. The extract with a concentration of 1000 ppm showed no significant difference with diclofenac Na at a concentration of 500 ppm (P ≥0.05). Based on the results of this research, it can be concluded that the aerial part of the C. citratus plant has anti-inflammatory activity and has the potential to be further developed as a candidate for anti-inflammatory drugs. Keywords: anti-inflammatory, 80% ethanol extract, lemongrass, Cymbopogon citratus, protein denaturation, Bovine Serum Album (BSA)