Electronic Theses and Dissertation

Sel punca mesenkimal (SPM) banyak dikembangkan potensinya untuk mengobati berbagai penyakit, hal ini dikarenakan SPM dapat berdiferensiasi menjadi berbagai jenis sel spesifik dan dengan mudah diperoleh dari jaringan dewasa, salah satunya darah tepi. Suara Al-Qur’an dapat memberikan efek terapeutik dengan merangsang hormon - hormon pertumbuhan yang penting dalam proses perbaikan jaringan. Penelitian ini menggunakan suara Al-Qur’an berupa murottal surat Al-Fatihah sebagai faktor eksternal yang merangsang diferensiasi sel punca mesenkimal asal darah tepi manusia menjadi sel saraf. Sampel darah diambil dari 3 orang laki-laki berusia 20-25 tahun, tidak merokok dan bebas HIV serta Hepatitis B. Sel punca mesenkimal diisolasi dengan metode gradien densitas menggunakan Fiqol. Sel punca mesenkimal diberi media diferensiasi saraf selama 21 hari dan diberi pemaparan suara Al-Qur’an selama 25 menit/hari. Hasil dianalisis berdasarkan hasil amplifikasi yang dilihat dengan nilai Ct pada qPCR menggunakan marka penanda saraf Nestin dan Tuj1 serta GAPDH sebagai gen kontrol. Hasil Analisis nilai Ct menunjukkan peningkatan ekspresi penanda saraf Nestin 1.5 x dan Tuj1 2.5 x lebih tinggi pada sel yang diberi pemaparan suara Al-Qur’an dibanding kontrol. Hasil ini menunjukkan suara Al-Qur’an berupa murottal Al-Fatihah berpotensi sebagai faktor eksternal dalam diferensiasi sel punca mesenkimal asal darah tepi manusia menjadi sel saraf.

Kata Kunci: Sel Punca Mesenkimal (SPM), Darah Tepi Manusia, Suara Al-Qur’an

Mesenchymal stem cells (MSCs) have been widely developed for their potential to treat various diseases, this is because MSCs can differentiate into multiple types of specific cells and are easily obtained from adult tissues, one of which is peripheral blood. The sound of the Qur'an can provide therapeutic effects by stimulating growth hormones that are important in the tissue repair process. This study used the sound of the Qur'an in the form of murottal of surah Al-Fatihah as an external factor that stimulates the differentiation of mesenchymal stem cells from human peripheral blood into neural cells. Blood samples were taken from 3 men aged 20-25 years, non-smokers and free of HIV and Hepatitis B. Mesenchymal stem cells were isolated by the density gradient method using Fiqol reagent. Mesenchymal stem cells were differentiated using a neural differentiation medium for 21 days and exposed to the sound of the Qur'an for 25 minutes/day. The results were analyzed based on the Ct value result in qPCR using the neural markers Nestin, Tuj1 and GAPDH as housekeeping gene. The results of the Ct value analysis showed that the expression of the neural markers Nestin increased 1.5 times and Tuj1 2.5 times higher in cells exposed to the sound of the Quran than in the control. These results indicate that the sound of the Qur'an, murottal Al-Fatihah has the potential as an external factor in the differentiation of mesenchymal stem cells from human peripheral blood into neural cells. Keywords: Mesenchymal stem cells (MSCs), Peripheral Blood, Al-Qur’an Sound