Electronic Theses and Dissertation

Sintesis monoasilgliserol (MAG) dan diasilgliserol (DAG) melalui reaksi gliserolisis RBDPKO menggunakan katalis enzim lipase yaitu Lipozyme TL IM dan Novozyme 435 telah berhasil dilakukan. Gliserolisis RBDPKO dilakukan dengan mereaksikan gliserol dan RBDPKO pada suhu 50℃ dengan rasio RBDPKO terhadap gliserol 1:2 (mol/mol) dengan jumlah katalis masing-masing sebesar 5% (b/v) dan variasi waktu reaksi selama 1, 2, 3, 4, 5, 6, 7, 8, 16 dan 24 jam. Kandungan MAG dan DAG dalam produk dianalisis menggunakan gas kromatografi. Hasil penelitian menunjukkan bahwa semakin lama waktu reaksi maka komposisi MAG dan DAG yang terbentuk semakin meningkat hingga diperoleh komposisi paling tinggi pada waktu reaksi optimumnya, di lain pihak komposisi TAG menurun. Penggunaan katalis Lipozyme TL IM menghasilkan 10,75% MAG dan 30,81% DAG pada waktu reaksi optimum 6 jam Penggunaan katalis Novozyme 435 menghasilkan 41,00% MAG dan 19,01% DAG pada waktu reaksi optimum 16 jam. Hasil penelitian ini menunjukkan bahwa penggunaan katalis Novozyme 435 pada reaksi gliserolisis RBDPKO menghasilkan kadar MAG yang lebih tinggi dibandingkan dengan penggunaan katalis Lipozyme TL IM.

The synthesis of monoacylglycerol (MAG) and diacylglycerol (DAG) through the RBDPKO glycerolysis reaction using lipase enzyme catalysts, namely Lipozyme TL IM and Novozyme 435, has been successfully carried out. Glycerolysis of RBDPKO was carried out by reacting glycerol and RBDPKO at a temperature of 50℃ with a ratio of RBDPKO to glycerol of 1:2 (mol/mol) with the amount of catalyst of 5% (b/v) each and variations in reaction time for 1, 2, 3, 4, 5, 6, 7, 8, 16 and 24 hours. The MAG and DAG content in the product was analyzed using gas chromatography. The research results showed that as the reaction time progressed, the composition of MAG and DAG increased until it reached optimum conditions, while the composition of TAG decreased. The use of Lipozyme catalyst produced 10.75% MAG and 30.81% DAG at an optimum reaction time of 6 hours. The use of Novozyme 435 catalyst produced 41.00% MAG and 19.01% DAG at an optimum reaction time of 16 hours. The results of the study showed that the use of the Novozyme 435 catalyst in the RBDPKO glycerolysis reaction produced higher MAG levels compared to the use of the Lipozyme TL IM catalyst.