Electronic Theses and Dissertation

Cinnamomum burmannii merupakan salah satu tumbuhan yang memiliki banyak manfaat baik itu sebagai obat maupun bahan pangan. Tumbuhan ini telah teruji mengandung berbagai aktivitas biologis seperti antioksidan, antiinflamasi, antidiabetes, analgetik, antitrombotik, antihiperglikemik, antimikroba, antijamur, dan antitumor. Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan menggunakan metode DPPH (1,1-diphenyl-2-picrylhydrazyl) dan antiinflamasi menggunakan metode aktivitas antiproteinase pada ekstrak diklorometana (DCM) daun C. burmannii. Daun C. burmannii diekstraksi dengan pelarut DCM sehingga diperoleh ekstrak DCM C. burmannii sebanyak 79,79 g (4,79%). Ekstrak DCM daun C. burmannii memiliki senyawa metabolit sekunder berupa alkaloid, flavonoid, tanin, fenol, steroid, terpenoid, dan saponin. Ekstrak DCM daun C. burmannii dipisahkan komponen-komponen kimianya menggunakan kromatografi kolom menghasilkan 5 sub fraksi, yaitu sub fraksi A-E diuji aktivitas antioksidan dan antiinflamasi. Hasil aktivitas antioksidan sampel yang paling kuat terdapat pada sub fraksi E dengan nilai IC50 0,001 ppm. Sementara aktivitas antiinflamasi sampel yang paling kuat terdapat pada sub fraksi A dengan nilai IC50 0,01 ppm.

Kata kunci: Cinnamomum burmannii, diklorometana, antioksidan, antiinflamasi, IC50

Cinnamomum burmannii is one of the plants that has a myriad of benefits both medicine and food. This plants has been tested to contain various biological activities such as antioxidant, anti-inflammatory, antidiabetic, analgetic, antithrombotic, antihyperglycemic, antimicrobial, antifungal, and antitumor. This study aims to determine the antioxidant acvtivity using the DPPH (1,1-diphenyl-2-picrylhydrazyl) method and its anti-inflammatory activity using the antiproteinase activity method in dichlorometana (DCM) extract of C. burmannii leaves. C. burmannii leaves was extracted using DCM to obtain 79,79 g (4,79%) of DCM extract of C. burmannii leaves. The DCM extract of C. burmannii leaves to obtain the chemical content of secondary metabolite compounds is alkaloids, flavonoids, tannin, phenol, steroids, terpenoids, and saponin. The DCM extract of C. burmannii leaves was separated from its chemical components using column chromatography to produce 5 sub fraction, namely sub fraction A-E were tested for antioxidant acvtivity using the DPPH method, and its anti-inflammatory activity using the antiproteinase activity method. The results of the strongest antioxidant activity of the sample was found in sub fraction E a with an IC50 value of 0,001 ppm. While the results of the strongest anti-inflammatory activity of the sample was found in sub fraction A a with an IC50 value of 0,01 ppm. Keywords: Cinnamomum burmannii, dichlorometana, antioxidant, anti-inflammatory, IC50