Electronic Theses and Dissertation

KONSENTRASI HORMON PROGESTERON PADA KUDA YANG DIDIAGNOSIS MENGALAMI PYOMETRA DI KABUPATEN
ACEH TENGAH
ABSTRAK
Populasi ternak kuda di Indonesia mengalami penurunan reproduksi yang disebabkan oleh beberapa penyakit dan gangguan reproduksi, salah satunya yaitu pyometra. Pyometra adalah infeksi rahim pada hewan betina yang ditandai dengan adanya nanah di dalam uterus dalam jumlah besar. Pyometra dapat ditandai dengan korpus luteum persisten yang bertahan lama dalam keadaan tidak normal, sehingga menyebabkan hormon progesteron meningkat dalam darah. Penelitian ini merupakan penelitian eksploratif lapangan yang bertujuan untuk mengamati konsentrasi hormon progesteron plasma darah kuda. Penelitian ini menggunakan sampel plasma darah yang dikoleksi dari tiga ekor kuda di Kabupaten Aceh Tengah yang didiagnosis mengalami pyometra. Sampel plasma yang diperoleh dilakukan pemeriksaan hormon progesteron menggunakan metode enzyme-linked immunosorbent assay (ELISA). Data yang diperoleh dianalisis secara deskriptif dan disajikan dalam bentuk Tabel. Hasil penelitian menunjukkan bahwa konsentrasi hormon progesteron kuda yang didiagnosis mengalami pyometra berkisar antara 12-39 ng/ml denga rataan 29,93±14,79 ng/ml. Konsentrasi hormon progesteron kuda1, 2 dan 3 berturut-turut adalah 12,85 ng/ml, 38,12 ng/ml dan 38,81 ng/ml. Berdasarkan hasil penelitian dapat disimpulkan bahwa kuda yang mengalami pyometra di Kabupaten Aceh Tengah memiliki konsentrasi hormon progesteron sekitar 12-39 ng/ml.
Kata kunci: ELISA, gangguan reproduksi, kuda, progesteron, pyometra.

PROGESTERON HORMONE CONCENTRATION IN HORSES WHO WAS DIAGNOSIS AS HAVING PYOMETRA IN CENTRAL ACEH DISTRICT ABSTRACT The equine population in Indonesia is experiencing a decline in reproduction caused by several diseases and reproductive disorders, one of which is pyometra. Pyometra is a uterine infection in female animals characterized by the presence of pus in the uterus in large quantities. Pyometra can be characterized by a persistent corpus luteum that persists for a long time in an abnormal state, causing the hormone progesterone to increase in the blood. This study is an exploratory field study that aims to observe the concentration of progesterone hormone in horse blood plasma. This study used blood plasma samples collected from three horses in Central Aceh District that were diagnosed with pyometra. Plasma samples obtained were examined for progesterone hormone using the enzyme-linked immunosorbent assay (ELISA) method. The data obtained were analyzed descriptively and presented in tables. The results showed that the progesterone hormone concentration of horses diagnosed with pyometra ranged from 12-39 ng/ml with an average of 29,93±14,79 ng/ml. The progesterone hormone concentrations of horses1, 2 and 3 were 12,85 ng/ml, 38,12 ng/ml and 38,81 ng/ml respectively. Based on the results of the study it can be concluded that horses suffering from pyometra in Central Aceh Regency have progesterone hormone concentrations around 12-39 ng/ml. Keywords: ELISA, horse, progesteron, pyometra, reproductive disruption