Electronic Theses and Dissertation

Spermatogenesis merupakan proses pembelahan dan diferensiasi sel dengan produk akhir spermatozoa yang berlangsung di sepanjang tubulus seminiferus testis. Selama spermatogenesis tepatnya saat spermiogenesis terjadi perkembangan akrosom spermatozoa yang melibatkan peran glikokonjugat dengan residu gula N-asetilgalaktosamin yang informasinya belum dilaporkan pada sapi aceh. Penelitian ini bertujuan mengetahui tahapan perkembangan akrosom selama spermiogenesis pada jaringan testis sapi aceh berdasarkan ekspresi N-asetilgalaktosamin yang dideteksi menggunakan metode histokimia lektin peanut agglutinin (PNA). Dalam penelitian ini digunakan lima organ testis sinister yang dikoleksi dari lima ekor sapi jantan dewasa berumur sekitar dua tahun. Sampel difiksasi dalam larutan neutral buffered formalin 10% lalu diproses menjadi preparat histologi dan diwarnai dengan pewarnaan lektin PNA metode avidin biotin complex peroxidase. Data tahapan perkembangan akrosom spermatozoa sapi aceh selama spermiogenesis dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa glikokonjugat jenis N-asetilgalaktosamin terdeteksi selama proses perkembangan akrosom, mulai dari round spermatid dan elongated spermatid yang berdiferensiasi menjadi spermatozoa. Perkembangan akrosom terjadi dalam 14 tahap (step) yang berlangsung dalam 8 tingkat (stage) tubulus seminiferus, yaitu tahap 1-7 terjadi pada stage I, tahap 8-9 pada stage II, tahap 10-11 pada stage III, tahap 12 pada stage IV, tahap 13 pada stage V dan VI, dan tahap 14 pada stage VII dan VIII. Berdasarkan fase spermiogenesis, steps 1-4 terjadi dalam fase Golgi, steps 5-7 dalam fase cap (tudung), steps 8-11 dalam fase akrosom, dan steps 12-14 dalam fase maturasi. Disimpulkan bahwa
perkembangan akrosom selama spermiogenesis pada sapi aceh berlangsung dalam 14 steps dari 8 stages tubulus seminiferus yang diidentifikasi berdasarkan ekspresi N-asetilgalaktosamin menggunakan lektin PNA.

Spermatogenesis is the process of cell division and differentiation with the final product being spermatozoa which takes place along the seminiferous tubules of the testicles. During spermatogenesis, specifically during spermiogenesis, the development of the spermatozoa acrosome occurs which involves the role of glycoconjugates with N-acetylgalactosamine sugar residues, information about which has not been reported in aceh cattle. This study aims to determine the stages of acrosome development during spermiogenesis in testicular tissue of aceh cattle based on the expression of N-acetylgalactosamine which was detected using the peanut agglutinin (PNA) lectin histochemical method. In this study, five left testicular organs were used which were collected from five adult bulls aged around two years. Samples were fixed in 10% neutral buffered formalin solution then processed into histology preparations and stained with PNA lectin staining using the avidin biotin complex peroxidase method. Data on the stages of acrosome development of aceh cattle spermatozoa during spermiogenesis were analyzed descriptively. The results showed that N-acetylgalactosamine glycoconjugates were detected during the acrosome development process, starting from round spermatids and elongated spermatids which differentiate into spermatozoa. Acrosome development occurs in 14 stages (steps) which take place in 8 levels (stages) of the seminiferous tubules, namely stages 1-7 occur in stage I, stages 8-9 in stage II, stages 10-11 in stage III, stage 12 in stage IV, stage 13 in stages V and VI, and stage 14 in stages VII and VIII. Based on the spermiogenesis phase, steps 1-4 occur in the Golgi phase, steps 5-7 in the cap phase, steps 8-11 in the acrosome phase, and steps 12-14 in the maturation phase. It was concluded that acrosome development during spermiogenesis takes place in 14 steps from 8 stages of seminiferous tubules aceh cattle identified based on N-acetylgalactosamine expression using the PNA lectin