Electronic Theses and Dissertation

Kanker payudara menjadi kanker dengan kasus tertinggi di Indonesia yang mengancam para wanita. Pogostemon cablin B. merupakan salah satu tanaman herbal endemic aceh yang telah diteliti terdapat potensi seperti antiinflamasi, anti proliferasi, antioksidan, antimikroba, dan proapoktosis. Untuk meneliti dan menganalisis kembali dilakukan eksplorasi melalui pendekatan Network pharmacology. Senyawa tanaman Pogostemon cablin B. didapatkan dari GCMS dan gen kanker payudara didapatkan dari OMIM, GeneCard, serta DisGeNet. Protein target serta alur yang berperan diidentifikasi menggunakan STRING-DB dan Metascape. Analisis terhadap jaringan dilakukan menggunakan Cytoscape. Didapatkan 65 senyawa tanaman dengan 554 protein target dan 1854 gen penyakit. Berdasarkan hasil analisis jaringan PPI didapatkan 138 protein tumpang tindih antara senyawa obat terhadap penyakit kanker payudara. Berdasarkan analisis KEGG dan GO protein pada Pogostemon cablin B. memiliki peran dalam mekanisme pengobatan/teurapatik kanker payudara. Berdasarkan mekanisme multi target “senyawa obat-protein target-Pathway” senyawa Pogostol, Isovalencenol, 1(2H)-Naphthalenone, octahydro-4a,8a-dimethyl-7-(1- methylethyl)-, [4aR-(4aa,7ß,8aa)]-,2(1H)Naphthalenone,3,5,6,7,8,8ahexahydro-4,8a-dimethyl-6-(1-methylethenyl)-, Cedran-diol, 8S,13-, Globulol. yang berinteraksi terhadap protein AKT1, EGFR, CCND1, PIK3CA, dan IGF1R pada jalur kanker secara umum, jalur kanker payudara, PI3K-Akt signaling Pathway, Proteoglycans in cancer, Pathways in cancer, FoxO signaling Pathway, dan HIF-1 signaling Pathway. Penelitian ini menjelaskan senyawa aktif, protein target, dan mekanisme alur dari pogostemin cablin terhadap pengobatan kanker payudara, melalui mekanisme multi-target dengan bahan alami.

Kata kunci: kanker payudara, Pogostemon cablin B., network pharmacology, AKT1, EGFR, Overall centrality

Breast cancer poses a significant threat to women in Indonesia, with breast cancer cases ranking highest among malignancies. Pogostemon cablin B., an endemic herbal plant in Aceh, has been studied for its potential anti-inflammatory, antiproliferative, antioxidant, antimicrobial, and proapoptotic properties. To further investigate and analyze, exploration was conducted using a Network Pharmacology approach. Plant compounds from Pogostemon cablin B. were identified through GCMS, while breast cancer-related genes were sourced from OMIM, GeneCard, and DisGeNet. Protein targets and Pathways were identified using STRING-DB and Metascape. Network analysis was performed using Cytoscape.A total of 65 plant compounds with 554 protein targets and 1854 disease-related genes were identified. The protein-protein interaction (PPI) analysis revealed 138 overlapping proteins between drug compounds and breast cancer-related proteins. Based on KEGG and GO analyses, proteins in Pogostemon cablin B. were found to play a role in the therapeutic mechanism of breast cancer. Utilizing a multi-target mechanism involving drug compounds, protein targets, and Pathways, specific compounds such as Pogostol, Isovalencenol, 1(2H)-Naphthalenone, octahydro-4a,8a-dimethyl-7-(1- methylethyl)-, [4aR-(4aa,7ß,8aa)]-,2(1H)Naphthalenone,3,5,6,7,8,8ahexahydro-4,8a-dimethyl-6-(1-methylethenyl)-, Cedran-diol, 8S,13-, Globulol. were identified to target significant proteins in the disease Pathway, including AKT1, EGFR, CCND1, PIK3CA, and IGF1R. This research elucidates the active compounds, protein targets, and Pathway mechanisms of Pogostemon cablin B. in breast cancer treatment through a multi-target mechanism utilizing natural ingredients. Keywords: breast cancer, Pogostemon cablin B., network pharmacology, AKT1, EGFR, Overall centrality