Electronic Theses and Dissertation

GAMBARAN HISTOPATOLOGIS HATI TIKUS PUTIH SPRAGUE DAWLEY JANTAN MODEL DIABETES MELLITUS TIPE 2 YANG DIINDUKSI STREPTOZOTOCIN

ABSTRAK

Diabetes melitus tipe 2 (DMT2) adalah bentuk paling umum dari diabetes yang salah satu komplikasinya dapat menyebabkan gangguan fungsi hati. Peningkatan kadar gula darah yang terjadi sebagai indikasi adanya DM Type 2 diperoleh melalui induksi streptozotocin (STZ). Penelitian ini bertujuan untuk mengetahui gambaran histopatologis hati tikus putih Sprague Dawley jantan sebagai model DM tipe 2 yang diinduksi streptozotocin (STZ). Metode penelitian dilakukan secara eksperimental murni menggunakan Rancangan Acak Lengkap (RAL) dengan tipe post-test only control group design. Tikus jantan strain Sprague Dawley yang digunakan berusia ±1,5 bulan dengan berat badan ±150 gram, dibagi menjadi kelompok: K+ dengan diet tinggi lemak, P1 dengan diet tinggi lemak dan STZ dosis 20 mg/kg BB, P2 dengan diet tinggi lemak dan STZ dosis 30 mg/kg BB, serta P3 dengan diet tinggi lemak dan STZ dosis 40 mg/kg BB. Pakan diet tinggi lemak diberikan selama 14 hari setelah tikus dilakukan aklimatisasi selama 7 hari. Selanjutnya tikus diperiksa kadar glukosa darah dan dilakukan induksi STZ sesuai perlakuan yang diinjeksikan secara intraperitoneal. Perlakuan dilakukan selama 56 hari dan dilakukan pengoleksian sampel hati untuk pembuatan preparat histopatologis. Data yang diperoleh dari pengamatan histopatologis berupa degenerasi lemak dan nekrosis dianalisis dengan analisis of variance (ANOVA) pola satu arah guna mengetahui perbedaan rata-rata yang dihasilkan antar kelompok perlakuan yang dilanjutkan dengan uji Duncan. Hasil rata-rata sel yang mengalami degenerasi pada perlakuan dengan dosis 0 mg, 20 mg, 30 mg dan 40 mg berturut-turut yaitu 32,00; 37,60; 38,80; dan 46,60 sementara nekrosis yaitu 19,20; 33,00; 37,20; dan 46,40. Hasil penelitian dapat disimpulkan bahwa induksi STZ dapat memengaruhi gambaran histopatologis hati tikus putih Sprague Dawley jantan dengan dosis 40 mg/kg BB sebagai dosis terbaik untuk induksi DMT2 (P

HISTOPATHOLOGICAL OVERVIEW OF THE LIVER IN MALE SPRAGUE DAWLEY RATS AS A TYPE 2 DIABETES MELLITUS MODEL INDUCED BY STREPTOZOTOCIN ABSTRACT Type 2 diabetes mellitus (T2DM) is known as the most common form of diabetes, with one of its complications leading to liver dysfunction.. Increased blood sugar levels, indicating the presence of T2DM, are obtained through the induction of streptozotocin (STZ). The histopathological features of the liver in male Sprague-Dawley rats as a model of STZ-induced T2DM are aimed to be determined in this study.. The research method was carried out using a pure experimental design with a completely randomized design (CRD) of the post-test only control group type. Male Sprague Dawley rats aged approximately 1,5 months with a body weight of approximately 150 grams were divided into groups: K+ with a high-fat diet, P1 with a high-fat diet and STZ at a dose of 20 mg/kg BW, P2 with a high-fat diet and STZ at a dose of 30 mg/kg BW, and P3 with a high-fat diet and STZ at a dose of 40 mg/kg BW. The high-fat diet was given for 14 days after the rats had acclimatized for 7 days. Next, the blood glucose levels of the rats were checked, and STZ induction was performed according to the treatments and injected intraperitoneally. The treatment was carried out for 56 days, and liver samples were collected for histopathological preparation. The data obtained from histopathological observations, in the form of fatty degeneration and necrosis, were analyzed using one-way analysis of variance (ANOVA) to determine the differences in the means between the treatment groups, followed by Duncan's test. The average numbers of cells undergoing degeneration in the treatments with doses of 0 mg, 20 mg, 30 mg, and 40 mg were found to be 32,00; 37,60; 38,80; and 46,60, respectively, while necrosis was observed to be 19,20; 33,00; 37,20; and 46,40, respectively. The study results concluded that the histopathological features of the liver in male Sprague Dawley rats can be affected by STZ induction, with the best dose for T2DM induction being 40 mg/kg BW (P