Electronic Theses and Dissertation

Luka bakar merupakan cedera akibat paparan zat kimia, aliran listrik dan suhu yang sangat tinggi. Luka bakar derajat II merupakan luka bakar ringan yang paling sering terjadi di lingkungan rumah tangga. Tanaman pala (Myristica fragrans) merupakan salah satu tanaman rempah yang secara empiris telah digunakan masyarakat untuk mengobati luka. Penelitian ini bertujuan untuk mengetahui efektivitas krim pala (Myristica fragrans) terhadap penyembuhan luka bakar derajat II pada kulit tikus putih. Penelitian ini menggunakan post-test only control group design terhadap 20 ekor tikus yang dibagi menjadi kelompok kontrol normal dan kelompok luka bakar derajat II yang diterapi sesuai kelompoknya. Pengolesan zat uji dilakukan dalam 18 hari, dua kali sehari. Pada hari ke 6 dan hari ke 18 kulit tikus dikoleksi dan dibuat menjadi slide yang diwarnai dengan HE. Selanjutnya, dilakukan penilaian pada jumlah fibroblas, jumlah angiogenesis dan pemeriksaan imunohistokimia score imunoekspresi TNF α. Hasil penelitian ini menunjukkan bahwa pemberian krim pala mampu menurunkan jumlah fibroblas dan jumlah angiogenesis pada kulit tikus yang diinduksi luka bakar yang berbeda signifikan dibandingkan tikus yang hanya diberikan basis krim (p=0,001) serta Hasil ini menunjukkan bahwa krim pala 3% dapat memperbaiki gambaran histopatologi kulit tikus yang diinduksi luka bakar derajat II serta mampu menurunkan score TNF α dibanding dengan yang hanya diberikan basis krim (p=0,021) .

Burns injury are injuries resulting from exposure to chemicals, electricity and extremely high temperatures. Second-degree burns are minor burns that most often occur in the household environment. Nutmeg (Myristica fragrans) is a spice plant that has been empirically used by the community to treat wounds. This study aims to determine the effectiveness of nutmeg cream (Myristica fragrans) on the healing of second degree burns on white rat skin. This study used a post-test only control group design on 20 rats divided into a normal control group and a second-degree burn group that was treated according to the group. The application of the test substance was carried out in 18 days, twice a day. On day 6 and day 18, the skin was collected and made into slides stained with Hematoxylin and Eosin (HE). Furthermore, the number of fibroblasts, the amount of angiogenesis and immunohistochemical examination of TNF α immunoexpression score were assessed. The results of this study showed that the administration of nutmeg cream was able to reduce the number of fibroblasts and the amount of angiogenesis in the skin of rats induced burns that were significantly different compared to rats that were only given a cream base (p = 0.001) and these results showed that 3% nutmeg cream could improve the histopathological picture of rat skin induced by second degree burns and was able to reduce TNF α score compared to those who were only given a cream base (p = 0.021).