Electronic Theses and Dissertation

ABSTRAK

Pemberian hormon PGF2α dapat meningkatkan kualitas spermatozoa melalui peningkatan kontraktilitas sel-sel otot polos pada jaringan testis. Peningkatan kontraktilitas sel-sel otot polos pada jaringan testis diduga dapat meningkatkan ekspresi protein Alpha-SMA. Penelitian ini bertujuan mengetahui distribusi dan peningkatan ekspresi Alpha-SMA pada organ testis tikus Wistar setelah pemberian PGF2α yang dideteksi menggunakan metode imunohistokimia (IHK). Penelitian ini menggunakan 15 tikus Wistar (Rattus norvegicus) jantan dan dibagi ke dalam 5 kelompok perlakuan (masing masing n=3) yakni kelompok kontrol (P0): koleksi testis dilakukan 30 menit setelah injeksi 0,5 NaCl 0,9%; kelompok 1 (P1): 30 menit setelah injeksi 2,5 mg PGF2α; kelompok 2 (P2): 60 menit setelah injeksi 2,5 mg PGF2α; kelompok 3 (P3): 90 menit setelah injeksi 2,5 PGF2α; dan kelompok 4 (P4): 120 menit setelah injeksi 2,5 mg PGF2α. Sampel testis diproses menjadi preparat histologi dan diwarnai dengan pewarnaan IHK metode avidin biotin complex peroxidase (ABC method). Distribusi Alpha-SMA dianalisis secara deskriptif, sedangkan perbedaan ekspresi Alpha-SMA dinilai dengan pemberian skor intensitas (intensity score) lalu hasilnya dianalisis menggunakan uji Kruskal-Wallis dan uji lanjut Mann Whitney U Test. Hasil penelitian menunjukkan bahwa Alpha-SMA terdeteksi positif pada sel myoid peritubular pada membran basal tubulus seminiferus, dinding buluh darah, dan jaringan ikat. Berdasarkan uji statistik, ekspresi Alpha-SMA pada sel myoid peritubular dan jaringan ikat testis tikus P2 (interval pemberian 60 menit) berbeda nyata dengan kelompok perlakuan lainnya (P0, P1, P3, dan P4) (P0,05). Disimpulkan bahwa pemberian PGF2α dapat meningkatkan ekspresi Alpha-SMA pada sel myoid peritubular dan jaringan ikat testis dengan interval waktu pemberian PGF2α terbaik adalah 60 menit.

Kata kunci: Alpha-SMA, IHK, PGF2α, testis dan tikus Wistar

ABSTRACT Administration of PGF2α hormone can improve the quality of spermatozoa by increasing the contractility of smooth muscle cells in testicular tissue. Increased contractility of smooth muscle cells in testicular tissue is thought to increase the expression of Alpha-SMA protein. This study aims to determine the distribution and increased expression of Alpha-SMA in the testicular organs of Wistar rats after administration of PGF2α detected using immunohistochemical (IHK) method. This study used 15 male Wistar rats (Rattus norvegicus) and divided into 5 treatment groups (each n=3), namely the control group (P0): testicular collection was performed 30 minutes after injection of 0.5 NaCl 0.9%; group 1 (P1): 30 minutes after injection of 2.5 mg PGF2α; group 2 (P2): 60 min after injection of 2.5 mg PGF2α; group 3 (P3): 90 min after injection of 2.5 PGF2α; and group 4 (P4): 120 min after injection of 2.5 mg PGF2α. Testicular samples were processed into histology preparations and stained with IHK staining avidin biotin complex peroxidase method (ABC method). The distribution of Alpha-SMA was analyzed descriptively, while the difference in Alpha-SMA expression was assessed by giving an intensity score and then the results were analyzed using the Kruskal-Wallis test and the Mann Whitney U Test. The results showed that Alpha-SMA was positively detected in peritubular myoid cells in the basement membrane of seminiferous tubules, blood vessel walls, and connective tissue. Based on statistical tests, the expression of Alpha-SMA in peritubular myoid cells and testicular connective tissue of P2 rats (60-minute administration interval) was significantly different from other treatment groups (P0, P1, P3, and P4) (P < 0.05) while in the blood vessel wall there was no significant difference between treatment groups (P>0.05). It is concluded that the administration of PGF2α can increase the expression of Alpha-SMA in peritubular myoid cells and testicular connective tissue with the best PGF2α administration time interval is 60 minutes. Key words: Alpha-SMA, IHK, PGF2α, testes, and Wistar rats