Electronic Theses and Dissertation

Bakteri merupakan mikroorganisme yang paling banyak terdapat di tanah. Penelitan ini bertujuan untuk mengetahui jumlah isolat bakteri yang berhasil diisolasi dari TPA Blang Bintang, mengetahui karakteristik makroskopik, mikroskopik, dan fisiologis (biokimia) bakteri asal TPA Blang Bintang, dan mengetahui kemampuan isolat bakteri asal TPA Blang Bintang sebagai antibakteri terhadap bakteri Staphylococcus aureus dan Escherichia coli. Parameter pada penelitian ini adalah jumlah isolat bakteri yang didapatkan, morfologi koloni bakteri (ukuran, warna, bentuk, tepian, dan elevasi), morfologi sel bakteri (bentuk sel dan pewarnaan Gram), karakter biokimia (katalase, motilitas, indol, H2S, urea, TSIA, MR-VP, dan sitrat) dan aktivitas antibakteri. Hasil penelitian menunjukkan terdapat 12 isolat bakteri yang terdiri dari bakteri Gram positif dan Gram negatif dengan bentuk basil, streptobasil, kokus, dan kokobasil. Kedua belas isolat bakteri tersebut secara umum memiliki morfologi yang beragam, yaitu berukuran titik dan kecil, berwarna putih, putih tulang, krem, dan krem kekuningan, berbentuk bundar dan tak beraturan, tepian licin, elevasi datar, seperti tombol, timbul, dan cembung. Hasil uji biokimia menunjukkan terdapat 8 genus bakteri, yaitu Bacillus (BTB 1, BTB 2, dan BTB 11), Planococcus (BTB 3), Staphylococcus (BTB 4, BTB 6, dan BTB 7), Enterococcus (BTB 5), Citrobacter (BTB 8), Pseudomonas (BTB 9), Erwinia (BTB 10), dan Acetobacter (BTB 12). Pada uji aktivitas antibakteri, isolat BTB 3, BTB 5, BTB 6, dan BTB 7 mampu menghambat bakteri Escherichia coli dan Staphylococcus aureus, sedangkan isolat BTB 1, BTB 4, BTB 8, dan BTB 10 hanya mampu menghambat bakteri Escherichia coli.

Bacteria are the most prevalent microorganisms in soil. This study aimed to determine the total bacterial isolates successfully isolated from Blang Bintang landfill, determine the macroscopic, microscopic, and physiology (biochemical) characteristics of bacteria from Blang Bintang landfill, and determine the ability of bacterial isolates from Blang Bintang landfill as antibacterial against Staphylococcus aureus and Escherichia coli bacteria. Parameters measured were the total bacterial isolates obtained, colony morphology (size, color, shape, edge, and elevation), cell morphology (cell shape and Gram staining), biochemical characters (catalase, motility, indole, H2S, urea, TSIA, MR-VP, and citrate) and antibacterial activity. The results showed that there were 12 bacterial isolates which consisted of Gram positive and Gram negative bacteria in the shape of bacilli, streptobacilli, coccus, and cocobacilli. The twelve isolates have varied morphology that is pinpoint and small size, white, bone white, cream, and yellowish cream colored, round and irregular shape, smooth edges, flat elevation, umbonate, raised, and convex. The results of the biochemical test indicate the presence of 8 bacterial genera, namely Bacillus (BTB 1, BTB 2, and BTB 11), Planococcus (BTB 3), Staphylococcus (BTB 4, BTB 6, and BTB 7), Enterococcus (BTB 5), Citrobacter (BTB 8), Pseudomonas (BTB 9), Erwinia (BTB 10), and Acetobacter (BTB 12). In the antibacterial activity test, isolates BTB 3, BTB 5, BTB 6, and BTB 7 were able to inhibit Escherichia coli and Staphylococcus aureus, while isolates BTB 1, BTB 4, BTB 8, and BTB 10 were only able to inhibit Escherichia coli.