Electronic Theses and Dissertation

Pemberian hormon PGF2α dapat meningkatkan kualitas semen melalui peningkatan konsentrasi hormon testosteron yang diduga dapat meningkatkan ekspresi reseptor androgen (RA) di dalam sel atau jaringan target dari hormon tersebut, terutama pada jaringan testis. Penelitian ini bertujuan mengetahui peningkatan ekspresi RA pada organ testis tikus Wistar setelah pemberian PGF2 yang dideteksi menggunakan metode imunohistokimia (IHK). Penelitian ini menggunakan 15 tikus Wistar (Rattus norvegicus) jantan yang dibagi ke dalam 5 kelompok perlakuan (masing-masing n=3). Kelompok kontrol (P0), koleksi testis dilakukan 30 menit setelah injeksi 0,5 ml NaCl 0,9%, sedangkan kelompok 1 (P1), kelompok 2 (P2), kelompok 3 (P3), dan kelompok 4 (P4), koleksi testis masing-masing dilakukan pada interval waktu 30 menit (P1), 60 menit (P2), 90 menit (P3), dan 120 menit (P4) setelah tikus diinjeksi dengan PGF2α sebanyak 2,5 mg/kg BB. Sampel testis diproses menjadi preparat histologi dan diwarnai dengan pewarnaan IHK metode avidin biotin complex peroxidase (ABC method). Sebaran RA dianalisis secara deskriptif, sedangkan perbedaan ekspresi RA dinilai dengan pemberian skor intensitas (intensity score) lalu hasilnya dianalisis menggunakan uji Kruskal-Wallis dan uji lanjut Mann Whitney U test. Hasil penelitian menunjukkan bahwa RA terdeteksi positif pada jaringan ikat, sel peritubular myoid, sel Leydig, sel Sertoli, dan buluh darah. Berdasarkan uji statistik terdapat perbedaan nyata ekspresi RA pada sel myoid peritubular dan sel Sertoli (P

The administration of PGF2α can improve semen quality through an increase in testosterone which is thought to increase androgen receptors (AR) in the target cells or tissues of the hormone, especially in testicular tissue. This study aims to determine the increase in RA expression in the testicular organs of Wistar rats after PGF2α administration that detected using immunohistochemical (IHC) method. This study used 15 male Wistar rats (Rattus norvegicus) which were divided into 5 treatment groups (n=3 each). The control group (P0), testes were collected 30 minutes after injection of 0.5 ml NaCl 0.9%. Group 1 (P1), group 2 (P2), group 3 (P3), and group 4 (P4), testes collection were carried out 30 minutes (P1); 60 minutes (P2), 90 minutes (P3), and 120 minutes (P4) after injection of 2.5 mg/kg BB PGF2α, respectively. Testicular tissue samples were processed into histology preparations and stained with avidin biotin complex peroxidase (ABC method) IHC staining. The distribution of AR was analyzed descriptively, while differences in AR expression were assessed by giving an intensity score and then the results were analyzed using the Kruskal-Wallis test and the Mann Whitney U test. The results showed that AR was detected positively in connective tissue, peritubular myoid cells, Leydig cells, Sertoli cells, and blood vessels. Based on statistical tests, there were significant differences in AR expression in peritubular myoid cells, and Sertoli cells (P