Electronic Theses and Dissertation

Monosodium glutamat (MSG) dihasilkan dari asam L-glutamat yang memiliki efek langsung pada tingkat seluler dengan membentuk radikal bebas yang menyebabkan stres oksidatif. Reactive oxygen spesies dan lipid peroksidase yang meningkat akibat stres oksidatif menyebabkan gangguan pada hormonal dan kualitas spermatozoa. Black garlic (BG) berfungsi sebagai antioksidan yang mampu menangani stres oksidatif. Penelitian dengan antioksidan lainnya telah banyak dilakukan, namun penggunaan BG untuk meningkatkan persentase gonadosomatic index (GSI), konsentrasi follicle stimulating hormone (FSH) dan kualitas spermatozoa pada tikus masih belum banyak diteliti. Penelitian ini merupakan metode kuantitatif dengan jenis penelitian rancangan acak lengkap dan terdiri dari 3 faktor, yaitu pemberian ekstrak BG 200 mg, 400 mg dan 600 mg, serta kelompok kontrol negatif dan kontrol positif dengan pemberian MSG. GSI dihitung dengan menggunakan rumus persentase perbandingan berat testis dengan berat badan, FSH menggunakan metode ELISA dari serum darah, dan kualitas spermatozoa dihitung menggunakan kamar hitung Neubauer dan pewarnaan eosin negrosin 2%. Hasil penelitian menunjukkan bahwa GSI tikus pada semua kelompok tidak berbeda signifikan (p>0,05). Konsentrasi FSH tikus menurun sangat signifikan pada kelompok kontrol positif dan meningkat sangat signifikan pada kelompok perlakuan dibanding kelompok kontrol negatif (p

Monosodium glutamate (MSG) is produced from L-glutamic acid which has a direct effect at the cellular level by forming free radicals that cause oxidative stress. Reactive oxygen spesies and lipid peroxidase which increases due to oxidative stress causing hormonal disturbances and spermatozoa quality. Black garlic (BG) functions as an antioxidant that is able to deal with oxidative stress. Much research has been done with other antioxidants, but BG is used to increase the percentage gonadosomatic index (GSI), concentration follicle stimulating hormone (FSH) and spermatozoa quality in mice have not yet been widely studied. This research is a quantitative method with a completely randomized design and consists of 3 factors, namely the administration of 200 mg, 400 mg and 600 mg BG extract, as well as a negative control group and a positive control group with the administration of MSG. GSI is calculated using the percentage formula comparing testicular weight to body weight, FSH using the ELISA method from blood serum, and spermatozoa quality is calculated using a counting chamber Neubauer and 2% eosin negrosine staining. The results showed that the GSI of mice in all groups was not significantly different (p>0.05). The FSH concentration of mice decreased very significantly in the positive control group and increased very significantly in the treatment group compared to the negative control group (p