Electronic Theses and Dissertation

Peningkatan testosteron dengan kualitas spermatozoa setelah pemberian PGF2α memiliki hasil yang tidak konsisten. Hasil yang tidak konsisten ini kemungkinan disebabkan perbedaan waktu pemeriksaan kualitas spermatozoa setelah pemberian PGF2α. Penelitian ini bertujuan mengetahui pengaruh perbedaan interval pemberian PGF2α dengan pengukuran konsentrasi testosteron terhadap peningkatan konsentrasi testosteron tikus Wistar. Dalam penelitian ini digunakan 12 tikus Wistar jantan yang diadaptasikan selama dua minggu dan dibagi ke dalam 4 kelompok (masing-masing n=3) yakni kelompok kontrol (P0), koleksi darah dilakukan 30 menit setelah injeksi 0,5 ml NaCl 0,9%; P1, koleksi darah dilakukan 30 menit setelah injeksi 2,5 mg/kg BB PGF2α; kelompok 2 (P2), koleksi darah dilakukan 60 menit setelah injeksi 2,5 mg/kg BB PGF2α; kelompok 3 (P3), dan kelompok 4 (P4), koleksi darah dilakukan 120 menit setelah injeksi 2,5 mg/kg BB PGF2α. Konsentrasi testosteron dalam plasma diukur menggunakan enzyme linked immunosorbent assay (ELISA). Data dianalisis dengan analisis varian satu arah dan uji Duncan. Konsentrasi testosteron pada kelompok P0; P1; P2; dan P3 masing-masing adalah 1,43±0,39; 1,05±0,49; 2,43±0,04; dan 1,34±0,38 ng/ml (P

Increasing testosterone and spermatozoa quality after administration of PGF2α has inconsistent results. These inconsistent results are likely due to differences in the timing of spermatozoa quality examination after administration of PGF2α. This study aims to determine the effect of differences in intervals between administering PGF2α and measuring testosterone concentrations on increasing testosterone concentrations in Wistar rats. In this study, 12 male Wistar rats were used which were adapted for two weeks and divided into 4 groups (n=3 each), namely the control group (P0), blood collection was carried out 30 minutes after injection of 0.5 ml of 0.9% NaCl; P1, blood collection was performed 30 minutes after injection of 2.5 mg/kg BW PGF2α; group 2 (P2), blood collection was carried out 60 minutes after injection of 2.5 mg/kg BW PGF2α; group 3 (P3), and group 4 (P4), blood collection was carried out 120 minutes after injection of 2.5 mg/kg BW PGF2α. Testosterone concentration in plasma was measured using enzyme linked immunosorbent assay (ELISA). Data were analyzed using one-way analysis of variance and Duncan's test. Testosterone concentration in the P0 group; P1; P2; and P3 were 1.43±0.39 respectively; 1.05±0.49; 2.43±0.04; and 1.34±0.38 ng/ml (P