Electronic Theses and Dissertation

Penelitian ini bertujuan untuk mengetahui potensi metabolit sekunder bioaktif yang terkandung dalam akar Rhizophora stylosa (G.). Kandungan senyawa bioaktif kualitatif dalam ekstrak diklorometana akar R. stylosa diskrining menggunakan metode fitokimia. Evaluasi antioksidan dilakukan dengan menggunakan uji 2,2-difenil-1-pikrilhidrazil; toksisistas–menggunakan metode Brine Shrimp Lethality Test (BSLT); dan antikanker– menggunakan uji 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) pada sel MCF-7. Ekstrak diklorometana dan fraksi gabungan yang terdiri dari RSD 1, RSD 2, RSD 3 dan RSD 4 menunjukkan aktivitas antioksidan dengan nilai IC50 berturut-turut yaitu sebesar 7,5 ppm, 214,2 ppm, 45,4 ppm, 85,6 ppm, dan 404,5 ppm. Toksisitas yang diperoleh berdasarkan nilai LC50 untuk ekstrak diklorometana akar R. stylosa dan fraksi gabungan RSD 1, RSD 2, RSD 3 dan RSD 4 berturut-turut yaitu 31,92 ppm, 531241,6 ppm, 332,48 ppm, 457,91 ppm, dan 3086,25 ppm. Serta ekstrak diklorometana akar R. stylosa dan fraksi gabungan RSD 1, RSD 2, RSD 3 dan RSD 4 menunjukkan aktivitas antikanker dengan nilai IC50 masing-masing sebesar 2198,50 ppm, 368,70 ppm, 225,50 ppm, 155,90 ppm dan 343,50 ppm. Penambatan molekuler tiga senyawa dominan yang terkandung di dalam ekstrak diklorometana, fraksi RSD 2 dan RSD 2.6.1 hasil kromatografi preparatif menunjukkan senyawa lupeol memiliki nilai energi ikatan paling besar terhadap reseptor ERα (-9,3 Kkal/mol), protein Bcl-2 (-7,1 Kkal/mol) dan Bcl-b (-6,9 Kkal/mol). Kesimpulannya, data aktivitas biologis menunjukkan potensi pengembangan ekstrak diklorometana akar R. stylosa dan fraksi gabungannya sebagai fitomedis untuk pengobatan kanker payudara.

Rhizophora stylosa (G.) roots contain bioactive secondary metabolites, and this investigation attempts to determine their therapeutic effects. Phytochemical techniques were applied to qualitatively screen the dichloromethane extract of R. stylosa roots for an abundance of bioactive compounds. The 2,2-diphenyl-1-picrylhydrazyl test was used to evaluate antioxidants; the Brine Shrimp Lethality Test technique was used to assess toxicity; and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) test was used to evaluate anticancer in MCF-7 cells. The dichloromethane extract and the total fraction which involves RSD 1, RSD 2, RSD 3, and RSD 4 demonstrated antioxidant activity, with respective IC50 values of 7,5 ppm, 214,2 ppm, 45,4 ppm, 85,6 ppm, and 404,5 ppm. Based on the LC50 values for the total fractions RSD 1, RSD 2, RSD 3, and RSD 4 and the dichloromethane extract of R. stylosa roots, respectively, the toxicity has been found to be 31.92 ppm, 531241.6 ppm, 332.48 ppm, 457.91 ppm, and 3086.25 ppm. Additionally, the R. stylosa root dichloromethane extract and all four fractions RSD 1, RSD 2, RSD 3, and RSD 4 demonstrated anticancer activity, with respective IC50 values of 2198.50 ppm, 368.70 ppm, 225.50 ppm, 155.90 ppm, and 343.50 ppm. Examine based on molecular docking of the three dominant compounds found in the dichloromethane extract, specifically fractions RSD 2 and RSD 2.6.1, the lupeol compound has the highest binding energy value to the ERα receptor (-9.3 Kcal/mol), Bcl-2 protein (-7.1 Kcal/mol), and Bcl-b (-6.9 Kcal/mol). In order to sum up, the data on biological activity suggests a dichloromethane extract of R. stylosa roots and its total fractions could be generated as a phytomedicine for the treatment of breast cancer.