Electronic Theses and Dissertation

Bromodosis adalah penyakit penyebab bau kaki yang disebabkan oleh bakteri Staphylococcus epidermidis dan Bacillus subtilis yang merupakan bakteri patogenik dan menjadi penyebab terbanyak terjadi Bromodosis. Tanaman pala (Myristica fragrans Houtt.) dari famili Myristicaceae yang berpotensi sebagai antibakteri. Tujuan penelitian ini untuk mengetahui aktivitas antibakterial minyak atsiri M. fragrans Houtt. terhadap bakteri Staphylococcus epidermidis dan Bacillus subtilis. Minyak atsiri M. fragrans Houtt. diperoleh melalui metode destilasi uap air. Uji aktivitas antibakterial dilakukan menggunakan metode difusi sumuran. Hasil karakteristik organoleptis, indeks bias dan kelarutan dalam etanol 90% menunjukkan bahwa minyak atsiri M. fragrans Houtt. sesuai dengan standar SNI 06-2388-2019, sementara uji berat jenis belum sesuai dengan standar yang telah ditentukan. Hasil uji aktivitas antibakterial dengan variasi konsentrasi minyak atsiri M. fragrans Houtt. 10; 20; 30; dan 40% terhadap Staphylococcus epidermidis dan Bacillus subtilis menunjukkan rata-rata diameter zona hambat mulai dari konsentrasi terkecil yaitu 10% sebesar 9,13 mm terhadap S.epidermidis dan 7,96 mm terhadap B. subtilis. Aktivitas terbesar konsentrasi 40% dengan zona hambat sebesar 13,33 mm terhadap S.epidermidis dan 11,36 mm terhadap B. subtilis. Hasil penelitian menunjukkan bahwa minyak atsiri M. fragrans Houtt. berpotensi sebagai agen antibakterial penyebab bromodosis.

Kata kunci : Bromodosis, Bacillus subtilis, Minyak atsiri M. fragrans Houtt. dan Staphylococcus epodermidis

Bromodosis is a disease that causes foot odor caused by the bacteria Staphylococcus epidermidis and Bacillus subtilis which are pathogenic bacteria and are the most common cause of Bromodosis. Nutmeg plants (Myristica fragrans Houtt.) from the Myristicaceae family have potential as antibacterials. The purpose of this study was to determine the antibacterial activity of M. fragrans Houtt. essential oil against Staphylococcus epidermidis and Bacillus subtilis bacteria. Essential oil of M. fragrans Houtt. was obtained through water vapor distillation method. The antibacterial activity test was conducted using the pitting diffusion method. The results of organoleptical characteristics, refractive index and solubility in 90% ethanol showed that M. fragrans Houtt. essential oil was in accordance with SNI 06-2388-2019 standards, while the specific gravity test was not in accordance with predetermined standards. The results of the antibacterial activity test with various concentrations of M. fragrans Houtt. 10; 20; 30; and 40% against Staphylococcus epidermidis and Bacillus subtilis showed the average diameter of the inhibition zone starting from the smallest concentration of 10% of 9.13 mm against S. epidermidis and 7.96 mm against B. subtilis. The largest activity was 40% concentration with inhibition zone of 13.33 mm against S.epidermidis and 11.36 mm against B. subtilis. The results showed that the essential oil of M. fragrans Houtt. has the potential as an antibacterial agent that causes bromodosis. Keywords: Bromodosis, Bacillus subtilis, Nutmeg essential oil. , Staphylococcus epodermidis