Electronic Theses and Dissertation

Penelitian ini bertujuan untuk mengetahui kualitas spermatozoa pasca thawing yang telah di berikan penambahan vitamin E dengan berbagai dosis. Penelitian ini bersifat eksperimental menggunakan rancangan acak lengkap (RAL) dengan 5 perlakuan dan 5 ulangan. Perlakuan penambahan dosis vitamin E dimasukkan ke dalam 100 ml pengencer skim kuning telur yang sudah di isi semen kecuali pada perlakuan kontrol (P0). Parameter yang diamati adalah persentase motilitas spermatozoa, persentase spermatozoa hidup, tudung akrosom utuh, dan membran plasma utuh. Data penelitian dianalisis dengan ANOVA menggunakan SPSS versi 18. Hasil penelitian menunjukkan bahwa penambahan vitamin E dengan dosis 0,15 g mendapatkan hasil persentase tertinggi diantara perlakuan lainnya. Berdasarkan hasil penelitian dapat disimpulkan bahwa penambahan vitamin E dengan dosis 0,15 g merupakan dosis efektif dan mampu mempertahankan kualitas spermatozoa dari kerusakan peroksidasi lipid dan cekaman suhu dingin.

This study aims to determine the quality of post-thaw spermatozoa which have been given the addition of vitamin E with various doses. This research is experimental using a completely randomized design (CRD) with 5 treatments and 5 replications. The treatment of adding a dose of vitamin E was put into 100 ml of egg yolk skim diluent which had been filled with semen except for the control treatment (P0). Parameters observed were the percentage of motility of spermatozoa, the percentage of live spermatozoa, intact acrosome cap, and intact plasma membrane. Research data were analyzed by ANOVA using SPSS version 18. The results showed that the addition of vitamin E at a dose of 0.15 g obtained the highest percentage results among other treatments. Based on the results of the study it can be concluded that the addition of vitamin E at a dose of 0.15 g is an effective dose and is able to maintain the quality of spermatozoa from damage to lipid peroxidation and cold temperature stress.