Electronic Theses and Dissertation

ABSTRAK


Ekstrak n-heksan daun sirsak (Anonna muricata) telah dilakukan uji toksisitas menggunakan metode Brine Shrimp Lethality Test (BSLT) terhadap 11 Fraksi dan 4 subfraksi kolom. Daun A. muricata dimaserasi menggunakan pelarut metanol dan dipartisi menggunakan pelarut n- heksan. Persen rendemen yang di peroleh ekstrak metanol (AMM) 15,99 % dan ekstrak n- heksan (AMH) 3,93 %. Sebanyak 11 kelompok fraksi yang diperoleh dari pemisahan ekstrak n- heksan daun A. muricata diuji toksisitasnya. Nilai LC50 yang diperoleh dari uji toksisitas dari 11 kelompok fraksi beturut-turut yaitu 1253,91; 4341,91; 57391,22; 1715,71; 1103,43; 12698,02;
587,70; 632,78; 644,89; 2138,78; 27,97 ppm. Toksisitas tertinggi diperoleh pada fraksi 11 dengan nilai LC50 27,97 ppm. Kemudian 4 kelompok subfraksi yang diperoleh dari pemisahan fraksi 11 didapatkan hasil uji toksisitas dengan nilai LC50 berturut-turut adalah 22,6; 14,2; 12,9; 6,41 ppm. Toksisitas tertinggi terdapat pada subfraksi 4 dengan nilai LC50 6,41 ppm. Kelompok subfraksi 4 dikarakterisasi menggunakan GC- MS menghasilkan 11 kelompoksenyawa dengan konsentrasi tertinggi yaitu senyawa monoterpen 6- Hydroxy- 4, 4, 7 a- trimethyl- 5, 6, 7, 7 a- tetrahydrobenzofurat- 2(4H)- one.

Kata kunci: Annona muricata, n-heksan, fitokimia, toksisitas, Brine Shrimp Lethality Test (BSLT), Lethal Concetration 50 (LC50), isolasi

ABSTRACT The n-hexane extract of soursop leaves (Anonna muricata) was tested for toxicity using the Brine Shrimp Lethality Test (BSLT) method on 11 fractions and 4 column subfractions. A. muricata leaves were macerated using methanol solvent and then partitioned using n-hexane solvent. The yield percentage obtained was 15,99% methanol extract (AMM) and 3,93% n-hexane extract (AMH). A total of 11 groups of fractions obtained from separating the n-hexane extract of A. muricata leaves were tested for their toxicity. The LC50 values obtained from the toxicity test of 11 successive fraction groups were 1253,91; 4341,91; 57391,22; 1715,71; 1103,43; 12698,02; 587,70; 632,78; 644,89; 2138,78; 27,97 ppm. The highest toxicity from the fraction 11 group was obtained in fraction 11 with an LC50 value of 27,97 ppm. Then the 4 subfraction groups obtained from the separation of fraction 11 obtained the results of the toxicity test with successive LC50 values of 22,6; 14,2; 12,9; 6,41 ppm. The highest toxicity test results were in the subfraction 4 group with an LC50 value of 6,41 ppm. Subfraction group 4 was characterized using GC-MS to produce 11 groups of compounds with the highest concentration, namely the monoterpene compound 6- Hydroxy-4,4,7a-trimethyl- 5,6,7,7a-tetrahydrobenzofurat-2(4H)-one. Keywords: Annona muricata, n-hexane, phytochemicals, toxicity, Brine Shrimp Lethality Test (BSLT), Lethal Concentration 50 (LC50), isolation