Electronic Theses and Dissertation

Bakteri pada ekosistem mangrove memiliki peran penting dalam proses penguraian. Tujuan penelitian ini adalah untuk menentukan jumlah populasi koloni bakteri yang terdapat di dalam tanah mangrove dan menentukan karakteristik makroskopis dan mikroskopis dari bakteri tanah mangrove. Metode pengambilan sampel pada penelitian ini dilakukan dengan purposive sampling. Sampel yang diambil dengan satu kali pengulangan pada tiga lokasi. Hasil penelitian menunjukkan bahwa dari 60 isolat yang ditemukan terdapat 7 isolat yang dimurnikan yaitu 4 isolat dari lokasi pertama, 2 isolat dari lokasi kedua dan 1 isolat dari lokasi ketiga. Jumlah populasi tertinggi terdapat pada lokasi pertama yaitu 1,6 x 105 CFU/g dan yang paling rendah adalah 0,10 x 107 CFU/g. Pengamatan secara makroskopis karakteristik tepi koloni terdapat 6 isolat berbentuk rata, 6 isolat berbentuk bulat, karakteristik elevasi koloni terdapat 6 isolat berbentuk melengkung dan 5 isolat berwarna krem berdasarkan pigmen koloni. Pengamatan mikroskopis meliputi bentuk sel, warna sel dan pewarnaan gram. Berdasarkan pengamatan mikroskopis memperlihatkan bahwa bentuk yang paling banyak ditemukan adalah basil sebanyak 5 isolat dan 2 isolat berbentuk kokus. Karakteristik pewarnaan gram terdapat 6 isolat gram positif dan 1 isolat gram negatif. Berdasarkan hasil penelitian, genus yang umum ditemukan adalah Bacillus sp. Micrococcus sp. Pseudomonas sp. Staphylococcus sp.


Kata Kunci: Bakteri, isolat, populasi, tanah, ekosistem mangrove

Bacteria in the mangrove ecosystem have an important role in the decomposition process. The purpose of were study was to determine the population of bacterial colonies found in mangrove soil and determine the macroscopic and microscopic characteristics of mangrove soil bacteria. The sampling method in this study was carried out by purposive sampling. Samples were taken with one repetition at three locations. The results showed that of the 60 isolates found, 7 isolates were purified, namely 4 isolates from the first location, 2 isolates from the second location and 1 isolate from the third location. The highest number of population was found in the first location, namely 1.6 x 105 CFU/g and the lowest was 0.10 x 107 CFU/g. From macroscopic observations of the characteristics of the edge of the colony, there were 6 flat-shaped isolates, 6 round-shaped isolates, and 6 isolates of colony elevation characteristics. curved shape and 5 isolates were cream colored based on colony pigments. Microscopic observations included cell shape, cell color and gram staining. Based on microscopic observations, it was shown that the most common forms found were bacilli with 5 isolates and 2 isolates in the form of cocci. Characteristics of gram staining, there were 6 gram positive isolates and 1 gram negative isolate. Based on the research results, the genus commonly found were Bacillus sp. Micrococcus sp. Pseudomonas sp. Staphylococcus sp. Keywords: Bacteria, isolates, population, soil, mangrove ecosystem