Electronic Theses and Dissertation

Sistem budidaya semi intensif merupakan sistem budidaya ikan lele dumbo (Clarias gariepinus) yang paling diminati oleh masyarakat di wilayah Aceh Besar, karena membutuhkan biaya produksi lebih sedikit akan tetapi sulit dalam pengontrolan pakan, pertumbuhan ikan dan penyakit. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi bakteri Vibrio sp. pada ikan lele dumbo yang di budidaya secara semi intensif. Penelitian ini dilakukan secara observasi lapangan, identifikasi Vibrio dengan menggunakan metode Carter, bakteri dikoleksi menggunakan media Alkaline Peptone Water (APW), selanjutnya diisolasi dan diidentifikasi dengan media selektif Thiosulfate Citrate Bile Salt Sucrose (TCBS), pewarnaan Gram dan uji biokimia. Hasil penelitian secara observasi menunjukkan bahwa kolam budidaya ikan lele berukuran standar untuk padat penebaran benih ikan serta memiliki kualitas air yang optimal. Hasil isolasi menunjukkan adanya pertumbuhan bakteri pada media APW dan terisolasi bakteri Vibrio pada media TCBS, yang berdasarkan uji biokimia menunjukkan Vibrio proteolyticus dan Vibrio fluvialis. Berdasarkan hasil penelitian ini disimpulkan bahwa ikan lele dumbo dari kolam budidaya semi intensif di Desa Beurangong Kecamatan Kuta Baro ditemukan adanya bakteri Vibrio proteolyticus, di Desa Dham Pulo Kecamatan Ingin Jaya Kabupaten Aceh Besar ditemukan adanya bakteri Vibrio fluvialis.

The semi-intensive farming system is dumbo catfish (Clarias gariepinus) farming system that is most in demand by people in the Aceh Besar, because it requires less production costs but is difficult to control feed, fish growth and disease. This study aims to isolate and identify Vibrio sp. in dumbo catfish that are semi-intensively. This study was conducted by field observations, identification of Vibrio were carried out using the Carter method, bacteria were collected using Alkaline Peptone Water (APW) media, then isolated and identified with Thiosulfate Citrate Bile Salt Sucrose (TCBS) selective media, Gram staining and biochemical tests. The results of observational research show that catfish farming ponds are standard sized for dense stocking of fish fry and have optimal water quality. The isolation results showed bacterial growth on APW media and isolated Vibrio bacteria on TCBS media, which based on biochemical tests showed Vibrio proteolyticus and Vibrio fluvialis. Based on the results of this study, it was concluded that dumbo catfish from semi-intensive aquaculture ponds in Beurangong Village, Kuta Baro, found the presence of Vibrio proteolyticus, in Dham Pulo Village, Ingin Jaya, Aceh Besar, Vibrio fluvialis were found.