Electronic Theses and Dissertation

Pengembangan perangkat mikroelektroda berbasis dielektroforesis (DEP) untuk karakterisasi sel darah merah telah banyak diteliti. Fabrikasi mikroelektroda dengan memanfaatkan material tembaga memberikan keuntungan berupa harga material dan biaya fabrikasi yang relatif murah. Penelitian ini bertujuan untuk menganalisis medan listrik, memfabrikasi, serta mengkarakterisasi sifat DEP sel darah merah pada mikroelektroda dengan struktur castellated. Medan listrik pada mikroelektroda castellated disimulasikan menggunakan software quick field 6.6 student. Mikroelektroda castellated difabrikasi menggunakan foil tembaga yang dilapisi di atas substrat kaca menggunakan teknik transfer pola. Karakterisasi DEP sel darah merah diuji dengan menerapkan sinyal AC pada mikroelektroda. Hasil penelitian menunjukkan distribusi medan listrik pada mikroelektroda castellated tidak seragam. Fenomena DEP sel darah merah terjadi pada mikroelektroda castellated yang berhasil difabrikasi dengan ukuran jarak antar elektroda dan jarak antar ujung elektroda 150 μm, 120 μm, dan 100 μm, dimana nDEP masing-masing terkarakterisasi pada rentang frekuensi 2,8 - 4,2 MHz, 2,4 - 4 MHz, dan 2,0 - 3,6 MHz serta pDEP masing-masing terkarakterisasi pada rentang frekuensi 5,8 - 7,2 MHz, 5,2 – 7 MHz, dan 5,0 - 6,6 MHz dengan tegangan listrik 4 - 8 Vpp dan konduktivitas listrik sampel 1,39 S/m.

The development of dielectrophoresis-based microelectrode devices (DEP) for the characterization of red blood cells has been widely studied. Microelectrode fabrication by utilizing copper material provides the advantage of relatively cheap material prices and fabrication costs. This study aims to analyze the electric field, fabricate, and characterize the DEP properties of red blood cells on microelectrodes with castellated structures. The electric field on the castellated microelectrode was simulated using the quick field 6.6 student software. Castellated microelectrodes were fabricated using copper foil coated on a glass substrate using a pattern transfer technique. RBC DEP characterization was tested by applying an AC signal to the microelectrode. The results showed that the electric field distribution on castellated microelectrodes was not uniform. The DEP phenomenon of red blood cells occurs in castellated microelectrodes that have been successfully fabricated with measurements of the distance between the electrodes and the distance between the electrode tips of 150 μm, 120 μm, and 100 μm, where each nDEP is characterized in the frequency range of 2.8 - 4.2 MHz, 2.4 - 4 MHz, and 2.0 - 3.6 MHz as well as pDEP each characterized in the frequency range 5.8 - 7.2 MHz, 5.2 - 7 MHz and 5.0 - 6.6 MHz with an electric voltage of 4 - 8 Vpp and a sample electrical conductivity of 1.39 S/m.