Electronic Theses and Dissertation

Nanopartikel perak (Ag0) menarik perhatian karena memiliki sifat antibakteri yang dapat digunakan secara luas. Pada penelitian ini, butiran silika gel (SiG) dilapisi dengan kitosan (chi) dan diimobilisasi dengan Ag0. Butiran silika gel yang dilapisi kitosan (chi- SiG) dikarakterisasi menggunakan Mikroskop dan SEM. Sintesis Ag0 menggunakan foto-iradiasi karena murah dan ramah lingkungan serta digunakan pelarut metanol untuk proses imobilisasi Ag0. Koloid chi-Ag0 dikarakterisasi menggunakan spektrofotometri UV-Vis dan FTIR. Spektrofotometer UV-Vis digunakan untuk mengamati surface plasmon resonance (SPR) nanopartikel perak pada 400-700 nm. Puncak serapan O-H dan N-H teramati pada 3424,76 cm-1 pada chi-Ag0 menunjukkan peran kitosan sebagai agen penstabil yang diamati melalui FTIR. Imobilisasi Ag0 pada [chi-SiG] dikarakterisasi menggunakan Mikroskop dan SEM. Permukaan silika gel ini berhasil dilapisi dengan kitosan dan diimobilisasi dengan Ag0. Butiran silika gel tidak menunjukkan adanya sifat antibakteri, namun material [chi-SiG] dan Ag0-[chi-SiG] menunjukkan aktivitas antibakteri yang baik melalui pengamatan zona hambat terhadap bakteri Staphylococcus aureus dan Escherichia coli. Pengujian lebih lanjut dilakukan terhadap bakteri berspora Bacillus subtilis dan hasil terbaik ditunjukkan oleh filter Ag0-[chi-SiG] sehingga sangat baik digunakan sebagai penyaring udara antibakteri untuk konfigurasi HEPA filter. Kata kunci: Nanopartikel perak, imobilisasi, aktivitas antibakteri, penyaring udara antibakteri, HEPA filter

Silver nanoparticles (Ag0) are particularly interested due to wide-ranging antibacterial properties. In this work, silikagel granules (SiG) were coated with chitosan (chi) and immobilized with Ag0. Silikagel granules coated with chitosan (chi-SiG) were characterized using Microscope and SEM. Synthesis of Ag0 used photo-irradiation because it is cheap and environmentally friendly and methanol solvent is used for the immobilization process of Ag0. Chi-Ag0 colloid was characterized using UV-Vis and FTIR spectrophotometry. UV-Vis was used to observe the surface plasmon resonance (SPR) of silver nanoparticles at 400-700 nm. Absorption peak of O-H and N-H was observed at 3424,76 cm-1 in chi-Ag0 indicating the function of chitosan as stabilizing agent which was observed through FTIR. Immobilization of Ag0 on [chi-SiG] was characterized using Microscope and SEM. Silikagel surface was successfully coated with chitosan and immobilized with Ag0. Silica gel granules did not show any antibacterial properties, but the [chi-SiG] and Ag0-[chi-SiG] materials showed good antibacterial properties by observing the inhibition zones against Staphylococcus aureus and Escherichia coli bacteria. Further tests were carried out on bacteria with the spore Bacillus subtilis and the best results was shown by the Ag0-[chi-SiG] filter, so it is very good to be used as an antibacterial air filter for HEPA filter configurations. Keywords: Silver nanoparticles, immobilization, antibacterial activity, antibacterial air filter, HEPA filter