Electronic Theses and Dissertation

GAMBARAN HISTOPATOLOGIS UTERUS KELINCI NEW ZEALAND WHITE BUNTING SEMU SETELAH MENGALAMI TRANSPLANTASI
DENGAN PERBEDAAN WAKTU LAMA OVARIUM
DI DALAM UTERUS
ABSTRAK
Salah satu dampak dari transplantasi adalah terjadinya stres (imunosupresif) yang menyebabkan sistem imun host akan menolak organ yang ditransplantasikan. Pada proses transplantasi ovarium pada uterus kelinci bunting semu, stres ini diduga akan menyebabkan kerusakan pada uterus kelinci bunting semu yang dapat disebabkan karena induksi bunting semu yang tidak terjadi secara sempurna dan lamanya ovarium berada di dalam uterus. Penelitian ini bertujuan mengetahui pengaruh peningkatan durasi ovarium di dalam uterus kelinci bunting semu selama proses transplantasi ovarium terhadap penurunan histopatologi pada uterus kelinci. Dalam penelitian ini digunakan sembilan ekor kelinci betina New Zealand White (NZW) bunting semu, berumur 3-5 tahun dan bobot badan 1,5-2,9 kg. Kelinci dibagi dalam tiga kelompok perlakuan setelah diadaptasikan selama 30 hari yaitu, K1 (n=3) kelompok kelinci yang dilakukan transplantasi ovarium selama 3 hari, K2 (n=3) kelompok kelinci yang dilakukan transplantasi ovarium selama 5 hari, dan K3 (n=3) kelompok kelinci yang dilakukan transplantasi ovarium selama 7 hari. Induksi bunting semu dilakukan dengan menggunakan PMSG dan hCG. Kelinci diinjeksi dengan 100 IU PMSG secara intramuskulus dan diikuti tiga hari kemudian dengan injeksi 75 IU hCG secara intravena. Transplantasi ovarium dilakukan pada hari ke-8 (Hari ke- 0 adalah hari ketika injeksi hCG). Uterus dikoleksi setelah proses transplantasi selesai dilakukan sesuai dengan kelompok perlakuan. Hasil penelitian menunjukkan bahwa pada K1, K2, dan K3 terlihat hiperemi pada epitel endometrium dan infiltrasi sel radang. Hiperemi dan nekrosis pada K1 cenderung lebih berat dibandingkan K2 dan K3. Jumlah hiperemi pada K1; K2; dan K3 masing-masing adalah 8,56±1,60; 5,78±2,15 dan 5,82±1,19 (P˂0,05), sedangkan Jumlah nekrosis masing-masing adalah 60,82±3,95; 56,98±4,95; 49,42±6,55 (P

HISTOPATHOLOGICAL FEATURES OF PSEUDOPREGNANT NEW ZEALAND RABBIT UTERUS AFTER TRANSPLANTATION WITH DIFFERENCES IN THE LENGTH OF TIME THE OVARIES ARE IN THE UTERUS ABSTRACT One of the effects of transplantation is the occurrence of stress (immunosuppressive) which causes the host immune system to reject the transplanted organ. In the process of ovarian transplantation in the uterus of pseudo pregnant rabbits, this stress is thought to cause damage to the uterus of pseudo pregnant rabbits which can be caused by pseudo bunting induction that does not occur completely and the length of time the ovaries are in the uterus. This study aims to determine whether increasing the duration of ovaries in the uterus of pseudo pregnant rabbits during the ovarian transplantation process can cause a decrease in histopathology in rabbit uterus. This study used nine female New Zealand White (NZW) pseudo pregnant rabbits, 3-5 years old and 1.5-2.9 kg body weight. Rabbits were divided into three treatment groups after 30 days of adaptation, namely, K1 (n=3) group of rabbits that performed ovarian transplantation for 3 days, K2 (n=3) group of rabbits that performed ovarian transplantation for 5 days, and K3 (n=3) group of rabbits that performed ovarian transplantation for 7 days. Induction of pseudo bunting was performed using PMSG and hCG. Rabbits were injected with 100 IU PMSG intramuscularly and followed three days later with an intravenous injection of 75 IU hCG. Ovarian transplantation was performed on day 8 (Day 0 was the day of hCG injection). Uterus was collected after the transplantation process was completed according to the treatment group. The results showed that in K1, K2, and K3 there was hyperemia in the endometrial epithelium and inflammatory cell infiltration. Hyperemia and necrosis in K1 tended to be heavier than K2 and K3. The hyperemia scores in K1; K2; and K3 were 8.56±1.60; 5.78±2.15 and 5.82±1.19 respectively (P>0.05), while the necrosis scores were 60.82±3.95; 56.98±4.95; 49.42±6.55 respectively (P