Electronic Theses and Dissertation

Penelitian ini bertujuan mengetahui potensi nefroproteksi ekstrak etanol kulit batang kayu Annona squamosa L. (EEKBAS) terhadap struktur histologis ginjal tikus (Rattus norvegicus Berk.) setelah diinduksi parasetamol dengan menghitung sel tubulus yang mengalami degenerasi dan nekrosis serta kerusakan hiperemi, hemoragi dan inflamasi. Rancangan yang digunakan adalah rancangan acak lengkap (RAL) yang terdiri dari 5 perlakuan dan 5 ulangan. Tikus kontrol normal diberi akuades selama 14 hari. Tikus kontrol negatif diberikan parasetamol dengan dosis 1.350 mg/kg BB. Pemberian ekstrak etanol kulit batang A. squamosa selama 14 hari dengan dosis 150 mg/kg BB, 300 mg/kg BB, dan 600 mg/kg BB, dan semua tikus diinduksi parasetamol 1.350 mg/kg pada hari ke-7, kecuali kontrol normal. Tikus dibedah pada hari ke-15, kemudian diambil organ ginjalnya untuk dibuat preparat histologi. Pembuatan sediaan histologis organ ginjal dilakukan menggunakan metode parafin dengan pewarnaan Hematoksilin dan Eosin (HE). Hasil analisis menunjukkan bahwa pemberian ekstrak etanol kulit batang Annona squamosa L. (EEKBAS) mampu menurunkan jumlah kerusakan sel tubulus ginjal yang mengalami degenerasi, nekrosis, hiperemi, hemoragi dan inflamasi akibat paparan parasetamol.

Kata kunci: Annona squamosa, kulit batang, parasetamol, nefroprotektor, degenerasi, nekrosis, hiperemi, hemoragi, inflamasi

This study aims to determine the nephroprotection potential of the ethanol extract of Annona squamosa L. stem bark (EEASSB) on the histological structure of the rat kidneys (Rattus norvegicus Berk.) after being induced by paracetamol by counting tubular cells that undergo degeneration and necrosis as well as hyperemia, hemorrhage and inflammation. The design used was a completely randomized design (CRD) consisting of 5 treatments and 5 replications. Normal control rats were given distilled water for 14 days. Negative control rats were given paracetamol at a dose of 1,350 mg/kg BW. Administration of ethanol extract of A. squamosa stem bark for 14 days at doses of 150 mg/kg BW, 300 mg/kg BW, and 600 mg/kg BW, and all rats were induced by paracetamol 1,350 mg/kg BW on day 7, except for normal controls. Mice were dissected on the 15th day, then their kidneys were taken to make histological preparations. Histological preparations of kidney organs were made using the paraffin method with Hematoxylin and Eosin (HE) staining. The results of the analysis showed that administration of ethanol extract of Annona squamosa L. stem bark (EEKBAS) was able to reduce the amount of damage to kidney tubular cells that experienced degeneration, necrosis, hyperemia, hemorrhage and inflammation due to exposure to paracetamol. Keywords: Annona squamosa, stem bark, paracetamol, nefroprotector, degeneration, necrosis, hyperemia, hemorrhage, inflammation.