Electronic Theses and Dissertation

ABSTRAK

Bakteri Staphylococcus aureus merupakan salah satu penyebab penyakit pada hewan dan manusia yang penanganannya sudah sulit dilakukan dengan antibiotik, sebagai upaya menemukan alternatif dari resistensi bakteri Staphylococcus aureus terhadap antibiotik. Penelitian ini bertujuan untuk mengetahui tingkat konsentrasi ekstrak etanol daun senggani yang memiliki daya hambat efektif terhadap bakteri Staphylococcus aureus secara in vitro. Maka pelaksanaan penelitian ini mengacu pada metode Kirby Bauer yang dimulai dengan reidentifikasi sederhana terhadap stok isolat bakteri Staphylococcus aureus menggunakan pewarnaan Gram dan uji katalase. Perlakuan penelitian menggunakan ekstrak etanol daun senggani dengan konsentrasi 25%, 50%, dan 75%, sebagai kontrol positif digunakan penisilin G dan kontrol negatif larutan CMC 1%. Hasil penelitian menunjukkan rata-rata diameter zona hambat pada konsentrasi 25% sebesar 16,08 mm, konsentrasi 50% sebesar 19,33 mm, 75% sebesar 20,57 mm, penisilin G sebesar 33,72 mm dan larutan CMC 1% sebesar 0 mm. Kesimpulan dari penelitian ini ekstrak etanol daun senggani memiliki daya hambat yang efektif terhadap pertumbuhan bakteri Staphylococcus aureus secara in vitro.


Kata kunci: Daya hambat, Faunus ater, Melastoma malabathricum L., penisilin G

ABSTRACT Staphylococcus aureus bacteria is one of the causes of disease in animals and humans, which are difficult to treat with antibiotics, therefore an effort to finded an alternative medicine to overcome the resistance of Staphylococcus aureus bacteria to antibiotics is necessarry. This study aimed to determine the concentration level of senggani leaf ethanolic extract, which has an effective inhibition against Staphylococcus aureus bacteria in vitro. The research was carried out according to the Kirby Bauer method, which began with simple reidentification of Staphylococcus aureus using Gram staining and catalase testing. The antibacterial test was conducted using senggani leaf ethanolic extract with a concentration of 25%, 50%, and 75%, penicillin G was used as a positive control, and CMC solution as a negative control. The results showed that the average diameter of the inhibition zone at 25% concentration was 16.08 mm, 50% concentration was 19.33 mm, 75% concentration was 20.57 mm, penicillin G was 33.72 mm, and CMC solution 0 mm. This study concludes that the ethanolic extract of senggani leaves effectively inhibits the growth of Staphylococcus aureus bacteria in vitro. Keywords: Inhibitory power, Faunus ater, Melastoma malabathricum L., penicillin G