Electronic Theses and Dissertation

Telah dilakukan penelitian tentang Analisis Kadar Formalin pada Tahapan Pembuatan
Ikan Keumamah, April-Juni 2022 di Laboratorium Kimia Laut dan Bioteknologi
Perikanan, Fakultas Kelautan dan Perikanan, Universitas Syiah Kuala. Penelitian ini
bertujuan untuk menganalisis kandungan formalin pada ikan segar hingga diolah
menjadi keumamah. Jenis ikan yang biasa diolah menjadi keumamah adalah ikan
Tongkol (Euthynus affinis). Analisis kadar formalin dilakukan dengan metode
spektrofotometri UV-vis menggunakan reagen Nash yang ditandai dengan perubahan
warna dari bening menjadi kuning. Kurva kalibrasi dibuat dari delapan konsentrasi
larutan formaldehid standar yaitu 0, 2, 4, 6, 8, 10, 12, 14 μg/mL. Berdasarkan hasil
penelitian diperoleh kadar formalin dari keempat ikan pada tiap-tiap perlakuannya.
Kadar formalin ikan sebelum dicuci, ikan setelah dicuci, ikan setelah direbus, dan ikan
setelah dikeringkan secara berturut berkisar antara 305,84-348,03 μg/m, 200,9-211,8
μg/mL, 155,55-173,44 μg/mL, dan 81,1-94,3 μg/mL. Ikan sebelum dicuci ke ikan
setelah dicuci terjadi penurunan kadar formalin sebanyak 95,6-147,13 μg/mL, ikan
setelah dicuci ke ikan setelah direbus terjadi penurunan kadar formalin sebanyak 38,8-
45,4 μg/mL dan ikan setelah direbus ke ikan setelah dikeringkan terjadi penurunan
sebanyak 62,53-79,92 μg/mL dan persentase penurunannya berkisar antara 60-75%,
78-84% dan 52-62%.

Kata Kunci :Euthynus affinis, formalin, konsentrasi, absorbansi, spektrofotometri UVvis.

Research of Analysis Formalin presence in the Production of Keumamah has been carried out from April to June 2022 at the Laboratory of Marine Chemistry and Fisheries Biotechnology, Faculty of Marine Sience and Fisheries, Syiah Kuala University. This study aims to analyze the formalin contentration in Tuna (Euthynus affinis) used as a raw material of Keumamah and the change of formalin concentration in eath step of Keumamah production. Analysis of formalin was carried out by the UV-vis spectrophotometry method using Nash reagent marked by a change in from clear to yellow in color. The calibration curves were contructed by preparing eight concentrations of standard formaldehyde solutions, namely 0, 2, 4, 6, 8, 10, 12, 14 μg/mL. Based on the research results for four sample, it was obtained that the average formalin concentration in the four fish in each treatment. The formalin levels of fish before washing, fish after washing, fish after boiling, and fish after drying ranged from 305.84-348.03 µg/mL, 200.9-211.8 µg/mL, 155.55-173.44 µg/mL, and 81.1-94.3 µg/mL. Fish before being washed to fish after washing decreased formalin levels by 95.6-147.13 µg/mL, fish after being washed on fish after boiling decreased formalin levels by 38.8-45.4 µg/mL and fish after boiling to fish after drying decreased by 62.53-79.92 µg/mL and the percentage of reduction ranged from 60-75%, 78-84% and 52-62%. Keywords : Euthynus affinis, formalin, concentration, absorbance, UV-vis spectrophotometry