Electronic Theses and Dissertation

Kakap Dori (Lutjanus fulviflamma) termasuk dalam famili Lutjanidae. Kakap memiliki nilai komersial yang tinggi di sebagian besar negara, termasuk Indonesia di kawasan Aceh. Ikan Kakap Dori termasuk salah satu spesies ikan kakap yang masuk dalam kategori sangat memprihatinkan keberadaannya di alam. Penelitian ini melaporkan adanya variasi genetik ikan ini di wilayah distribusinya dengan memanfaatkan gen penanda DNA mitokondria COI. Pengambilan sampel ikan di Aceh dikumpulkan dari tempat pendaratan ikan di Pelabuhan Perikanan Samudera (PPS) Lampulo, Banda Aceh. Seluruh sekuen L. fulviflamma yang berasal dari daerah lain di wilayah distribusinya diunduh dari GenBank melalui situs NCBI dan BOLD Systems dan kemudian diperiksa secara bersamaan untuk melihat Komposisi Basa Nukleotida, konektivitas spesies, pola filogeografi, dan jarak genetik. Seluruh sekuen yang diperoleh diedit dan diselaraskan menggunakan MEGA X. Selanjutnya, DnaSP 5.10 digunakan untuk memperoleh persebaran haplotipe. Selain itu, Minimum Spanning Network (MSN) versi 10.3 digunakan untuk menghasilkan representasi grafis dari hubungan haplotipe. Secara keseluruhan, delapan puluh enam sekuen digunakan dalam penelitian ini (Aceh: 12; Genbank dan BOLD: 74) dan empat puluh lima haplotipe dihasilkan dalam penelitian ini. Nilai komposisi basa nukleotida dari populasi ikan kakap dori yang berasal dari Utara Aceh didominasi oleh basa AT (52,69%). Keanekaragaman haplotipe tertinggi tercatat pada 9 populasi (Bali, Surabaya, Iran, Seychelles, Filipina, Oman, Bangladesh, Vietnam, dan Malta), dengan nilai 1, sedangkan terendah tercatat di Australia dengan nilai 0. Jarak genetik antar populasi tertinggi terdapat pada populasi antara Aceh Utara dan India (0,021), sedangkan nilai jarak genetik tertinggi pada populasi terdapat pada populasi India sebesar 0,017

Kata kunci : Kakap, Perairan Aceh, Pendaratan Ikan, Sekuen

The Dory snapper (Lutjanus fulviflamma) belongs to the family Lutjanidae. The snapper is commercially fish commodity for some countries, including Indonesia in the Aceh area. The Dory snapper is one of the snapper species whose existence in nature is least concern. This reaserch report the genetic variation of this fish in its distribution area utilizing the mitochondrial DNA COI gene. The fish samples in Aceh were gathered from fish landing in Pelabuhan Perikanan Samudra (PPS) Lampulo, Banda Aceh. Additional sequences of L. fulviflamma from other areas in its distribution region were downloaded from GenBank at the NCBI and the BOLD Systems website and then examined simultaneously to see the nucleotide base composition, species connectivity, phytogeography pattern, and genetic distance. All obtained sequences were edited and aligned using MEGA X. Furthermore, DnaSP 5.10 was used to summarize haplotype distributions. In addition, Minimum Spanning Network (MSN) version 10.3 was used to generate a graphical representation of haplotype relationships. 86 sequences were used in this study (Aceh: 12; GenBank and BOLD: 74) and 45 haplotypes were generated in this study. The nucleotide base composition of the dori snapper population from northern Aceh is dominated by AT (52,29%). The highest haplotype diversity was recorded in 9 populations (Bali, Surabaya, Iran, Seychelles, Philippines, Oman, Bangladesh, Vietnam, and Malta), with a value of 1, while the lowest was recorded in Australia with 0 value. The highest genetic distance between populations was found in the population between North Aceh and India (0.021), while the highest genetic distance value in the population is in the India population of 0.017 Keywords: Snappers, Aceh waters, Fish landing, Sequence