Electronic Theses and Dissertation

Abstrak. Tujuan penelitian ini adalah untuk mengetahui pengaruh jenis isolat rizobakteri asal Kecamatan Kaway XVI Kabupaten Aceh Barat terhadap pertumbuhan patogen Phytophthora capsici dan peranannya sebagai pemacu pertumbuhan tanaman secara in vitro. Penelitian ini berlangsung di Laboratorium Ilmu dan Teknologi Benih, Jurusan Agroteknologi, Fakultas Pertanian, Universitas Syiah Kuala, Darussalam Banda Aceh dan Laboratorium Biologi, Jurusan Pendidikan Biologi, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Syiah Kuala, Darussalam Banda Aceh. Penelitian ini dimulai pada November sampai dengan Desember 2022. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) non faktorial yang terdiri dari 15 perlakuan rizobakteri dan diulang sebanyak 3 kali. Hasil penelitian menunjukkan bahwasannya pengujian untuk mengetahui jenis rizobakteri yang mempunyai daya hambat pertumbuhan koloni patogen Phytophthora capsici memberikan pengaruh yang sangat nyata baik terhadap persentase daya hambat maupun laju penghambatan pertumbuhan P. capsici. Isolat BPS 8/4 memiliki penghambatan tertinggi yakni 79,92%. Sedangkan laju penghambatan pertumbuhan patogen tertinggi dimiliki isolat BPS 7/1 dan BPS 8/2 dengan nilai 3,75 mm/hari. Isolat TPS 6/1 dengan produksi HCN tinggi (+++ HCN) yakni isolat yang mampu menghasilkan HCN. Kemampuan rizobakteri untuk menghasilkan IAA dan melarutkan fosfat menunjukkan fungsinya sebagai pemacu pertumbuhan tanaman. Produksi IAA lebih tinggi pada isolat rizobakteri TPS 6/3 dengan nilai rata-rata 0,721 µg/ml filtrat.
Kata Kunci: In vitro, P. capsici, Rizobakteri

Abstract. The purpose of this study was to determine the effect of the type of rhizobacterial isolates from Kaway XVI District, West Aceh Regency on the growth of the pathogen Phytophthora capsici and its role as a promoter of plant growth in vitro. This research took place at the Seed Science and Technology Laboratory, Department of Agrotechnology, Faculty of Agriculture, Syiah Kuala University, Darussalam Banda Aceh and the Biology Laboratory, Department of Biology Education, Faculty of Teaching and Education, Syiah Kuala University, Darussalam Banda Aceh. This study started from November to December 2022. This study used a non-factorial Completely Randomized Design (CRD) which consisted of 15 rhizobacterial treatments and was repeated 3 times. The results showed that the test to determine the type of rhizobacteria that had the ability to inhibit the growth of the pathogen Phytophthora capsici colonies had a very significant effect on both the percentage of inhibition and the growth inhibition rate of P. capsici. BPS 8/4 isolate had the highest inhibition, namely 79.92%. Meanwhile, the highest inhibition rate of pathogen growth was found in BPS 7/1 and BPS 8/2 with a value of 3.75 mm/day. TPS 6/1 isolate with high HCN production (+++ HCN) are isolates capable of producing HCN. The ability of rhizobacteria to produce IAA and dissolve phosphate indicates its function as a plant growth promoter. IAA production was higher in TPS 6/3 rhizobacterial isolate with an average value of 0.721 µg/ml filtrate. Keywords: In vitro, P. capsici, Rhizobacteria