Electronic Theses and Dissertation

Stem Cell menjadi salah satu teknologi yang digunakan dalam upaya penyembuhan penyakit
degeneratif seperti osteoporosis. Penelitian ini bertujuan untuk mengetahui pengaruh suara
murottal Al-Fatihah sebagai faktor eksternal dalam proliferasi dan diferensiasi Mesenchymal
Stem Cell (MSC) asal darah tepi, serta transdiferensiasi menjadi seperti sel tulang. Penelitian ini
dilakukan di Laboratorium Struktur dan Perkembangan Jurusan Biologi FMIPA Universita s
Syiah Kuala, mulai dari bulan Februari sampai November 2022. Metode penelitian yang
digunakan yaitu metode Rancangan Acak Lengkap (RAL) dengan 5 perlakuan dan 3 ulangan.
Perlakuan berupa pemaparan suara murottal Al-Fatihah dengaan durasi yang berbeda-beda
diantaranya yaitu P0 (0 menit/hari), P1 (1,47 menit/hari), P2 (2,41 menit/hari), P3 (3,36
menit/hari), P4 (13,32 menit/hari), dan P5 (15,20 menit/hari). Parameter pada penelitian ini
adalah proliferasi sel yang dihitung menggunakan metode Population Doubling Time (PDT) dan
transdiferensiasi seperti sel tulang yang dihitung menggunakan persentase sel. Hasil yang
diperoleh pada penelitian ini berdasarkan Uji Non-Parametrik (Kruskal Wallis) diperoleh nilai
P(0,03) < 0.05, dengan demikian pemaparan murottal Al-Fatihah berpengaruh terhadap tingkat
proliferasi sel. Nilai PDT tercepat yaitu pada perlakuan P5 (10,23 jam). Pemaparan murottal AlFatihah juga berpengaruh terhadap jumlah transdiferensiasi seperti sel tulang, dengan nilai yang
diperoleh berdasarkan Analisis Varian (ANOVA) yaitu P(0,00) > 0.05, perlakuan yang paling
berpengaruh secara berurut adalah P5 (27.20%), P4 (23,84%), P3 (18,87%), P2 (15,01%), P1
(8,65%), dan P0 (6,44%). Hasil penelitian ini dapat diaplikasikan dalam upaya terapi
penyembuhan penyakit degeneratif.
Kata Kunci : Mesenchymal Stem Cell (MSC), proliferasi, transdiferensiasi, murottal Al-Fatihah.

Stem cell theraphy is one of the technologies being used to treat degenerative diseases such as osteoporosis. The purpose of this study is to determine the effect of Al-Fatihah murottal sound as an external factor in the proliferation and differentiation of Mesenchymal Stem Cell (MSC) from peripheral blood, as well as transdiferentiation into bone-like cell. This research was conducted at the Structure and Development Laboratory of the Department of Biology, Faculty of Mathematics and Natural Sciences, Syiah Kuala University, f rom February to November 2022. The research method used was the Complete Randomized Design method with 5 treatments and 3 replications. The treatment in the form of exposure to murottal Al-Fatihah include P0 (0 minutes/day), P1 (1,47 minutes/day), P2 (2,41 minutes/day), P3 (3,36 minutes/day), P4 (13.32 minutes/day), P5 (15,20 minutes/day), and P6 (24,27 minutes/day).The parameters in this study were cell proliferation which was calculated using the Population Doubling Time (PDT) method, and transdifferentiation such bone cells like which was calculated using the percentage of cell counts. The results obtained in this study based on the Non-Parametric Test (Kruskal Wallis) obtained a value of P(0.03) < 0.05, thus exposure to murottal Al-Fatihah affects the rate of cell proliferation. The fastest PDT value is in the P5 treatment (10,23 hours). Exposure to murottal Al-Fatihah also affect the amount of transdifferentiation such as bone cells, with the value obtained based on the Analysis of Variance (ANOVA) that is P(0.00) < 0.05, the most influential treatment sequentially is P5 (27,20%), P4 (23,84%), P3 (18,87%), P2 (15,01%), P1 (8,65%), and P0 (6,44%). The results of this research can be potentially applied in efforts to cure degenerative diseases. Keywords : Mesenchymal Stem Cell (MSCs), proliferation, transdifferentation, murottal Al-Fatihah.