Electronic Theses and Dissertation

Alpha smooth muscle actin (α-SMA) merupakan salah satu protein yang berkontribusi terhadap kontraksi otot polos. Penelitian ini bertujuan untuk mengetahui variasi distribusi dan ekspresi α-SMA pada kaput, korpus dan kauda epididimis sapi aceh yang dideteksi dengan teknik imunohistokimia (IHK). Penelitian ini menggunakan empat pasang organ epididimis sapi aceh jantan dewasa berumur 2-3 tahun yang dipisahkan menjadi bagian kaput, korpus, dan kauda lalu diproses menjadi preparat histologi dan diwarnai dengan pewarnaan IHK metode avidin biotin complex peroxidase. Distribusi α-SMA pada kaput, korpus, dan kauda epididimis dianalisis secara deskriptif sedangkan ekspresi α-SMA dinilai dengan pemberian skor intensitas (intensity score). Data yang diperoleh dianalisis menggunakan uji Kruskal - Wallis dan dilanjutkan dengan uji Mann Whitney U Test. Ketebalan otot polos pada kaput, korpus, dan kauda epididimis diukur dengan software ToupView, data dianalisis menggunakan uji ANOVA dan dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan α-SMA terdeteksi positif pada kaput, korpus, dan kauda epididimis, yaitu pada membran basal, jaringan ikat, buluh darah, dan sel otot polos, tetapi tidak ditemukan pada lapisan epitel. Uji statistik memperlihatkan tidak terdapat perbedaan ekspresi α-SMA pada membran basal, jaringan ikat, buluh darah, dan sel otot polos diantara bagian kaput, korpus, dan kauda epididimis (P>0,05). Lapisan otot polos yang mengekspresikan α-SMA pada bagian kaput (11,42±1,64 µm), korpus (20,93±6,75 µm), dan kauda (58,82±6,43 µm), secara statistik berbeda nyata (P

Alpha smooth muscle actin (α-SMA) is a protein that contributes to smooth muscle contraction. The objective of this study was to determine the variation of distribution and expression of α-SMA in the caput, corpus, and cauda epididymis of aceh cattle detected by immunohistochemical (IHC) techniques. This study used four pairs of the epididymis of adult aceh cattle aged 2-3 years. The epididymis was separated into the caput, corpus, and cauda and then processed into histological preparations and stained with IHC staining. The distribution of α-SMA in caput, corpus, and cauda epididymis was analyzed descriptively, while the expression of α-SMA was assessed by giving the intensity score. The data obtained were analyzed using the Kruskal - Wallis test and continued with the Mann Whitney U Test. Additionally, smooth muscle thickness in caput, corpus, and cauda epididymis was measured using ToupView software and the measurement data were analyzed using ANOVA test and continued with Duncan test. The results showed that α-SMA was positively detected in the caput, corpus, and cauda epididymis, namely basement membrane, connective tissue, blood vessels, and smooth muscle cells, however, it was not found in the epithelial layer. Based on the results of statistical tests, there was no difference in the expression of α-SMA in the basement membrane, connective tissue, blood vessels, and smooth muscle cells between the caput, corpus, and cauda epididymis (P>0.05). Smooth muscle layers expressing α-SMA in the head (11.42±1.64 µm), corpus (20.93±6.75 µm), and cauda (58.82±6.43 µm), were statistically significantly different (P