Electronic Theses and Dissertation

Penelitian ini bertujuan untuk mengukur aktivitas antioksidan, mengkarakterisasi sifat biokimia dan melakukan identifikasi molekuler gen 16S rRNA pada isolat BETD5. Metode yang digunakan pada penelitian ini adalah metode eksperimental. Parameter yang diamati adalah aktivitas antioksidan yang terdiri dari IC50 (Inhibition Consentration), sifat biokimia serta pohon filogenetik gen 16S rRNA. Isolat yang digunakan adalah ekstrak bakteri endofit tapak dara (BETD5) dengan variasi konsentrasi ekstak sampel yaitu 5ppm, 10ppm, 25ppm, 50ppm dan 100ppm. Uji biokimia yang dilakukan meliputi uji Triple Sugar Iron Agar (TSIA), uji Simmons Citrate (SC), uji motilitas, uji indol, uji katalase dan uji Methyl Red (MR). Identifikasi molekuler gen 16S rRNA dilakukan untuk mengetahui kekerabatan dengan organisme lain melalui pohon filogenetik. Hasil penelitian menunjukkan bahwa aktivitas antioksidan isolat BETD5 kuat karena IC50 yang diperoleh sebesar 78,485 ppm. Berdasarkan uji biokimia, isolat BETD5 diduga merupakan genus Staphylococcus. Berdasarkan hasil BLAST isolat BETD5 memiliki kemiripan dengan Staphylococus gallinarium strain VIII 1 sebesar 99,38%.

Kata kunci: Antioksidan, bakteri endofit, gen 16S rRNA, sifat biokimia.

The aims of this study were to measure antioxidant activity, to characterize its biochemical properties and to identify the 16S rRNA gene in the isolates of BETD5 molecularly. The method used in this research is the experimental method. Parameters observed were antioxidant activity consisting of IC50 (Inhibition Concentration), biochemical properties and phylogenetic tree of 16S rRNA gene. The isolates used were endophytic bacteria tapak dara (BETD5) with varying sample concentrations, namely 5ppm, 10ppm, 25ppm, 50ppm and 100ppm. The biochemical tests carried out included the Triple Sugar Iron Agar (TSIA) test, Simmons Citrate (SC) test, motility test, indole test, catalase test and Methyl Red (MR) test. Molecular identification of the 16S rRNA gene was carried out to determine the relationship with other organisms through a phylogenetic tree. The results showed that the antioxidant activity of the BETD5 isolate was strong because the IC50 obtained was 78,485 ppm. Based on biochemical tests, the isolate BETD5 was suspected to be the genus Staphylococcus. Based on the BLAST results, the isolate BETD5 was 99,38% similar to Staphylococus gallinarium strain VIII 1. Keywords: Antioxidant, endophytic bacteria, 16S rRNA gene, biochemical properties.