Electronic Theses and Dissertation

Abstrak teknologi stem cell memiliki potensi dalam upaya penyembuhan penyakit degeneratif. penelitian bertujuan untuk mengetahui ekstrak nilam (pogostemon cablin benth) sebagai media penginduksi terhadap proliferasi dan transdiferensiasi mesenchymal stem cell (msc) asal darah tepi menjadi sel saraf. penelitian dilaksanakan di laboratorium struktur dan perkembangan a jurusan biologi fakultas matematika dan ilmu pengetahuan alam universitas syiah kuala dari bulan januari sampai bulan agustus 2021. metode penelitian menggunakan metode rancangan acak lengkap (ral) dengan 7 perlakuan dan 3 ulangan. perlakuan berupa penambahan ektrak nilam (p. cablin) yang terdiri dari p0 (kontrol positif dengan media stemdiff forebrain neuron differentiation kit), p1 (kontrol negatif menggunakan media dulbecco’s modified eagle’s medium (dmem) tanpa penambahan ekstrak), p2 (dmem + 3 mg/ml esktrak nilam), p3 (dmem + 5 mg/ml esktrak nilam), p4 (dmem + 7 mg/ml esktrak nilam), p5 (dmem + 9 mg/ml esktrak nilam), dan p6 (dmem + 11 mg/ml esktrak nilam). parameter penelitian adalah proliferasi yang dihitung menggunakan rumus population doubling time (pdt) dan transdiferensiasi sel saraf yang dihitung menggunakan persentase jumlah sel. hasil penelitian menunjukkan bahwa ekstrak nilam (p. cablin) memberikan pengaruh tingkat proliferasi yang sama pada setiap perlakuan yang diujikan. nilai population doubling time (pdt) tercepat dicapai oleh perlakuan p0 (223,2 jam) dan p4 (321,6 jam), serta ekstrak nilam berpengaruh terhadap jumlah transdiferensiasi sel saraf (p

ABSTRACT Stem cell technology has a great potential to cure degenerative disease. This study aimed to determine the potential induction of Pogostemon cablin Benth extract to proliferation and differentiation of peripheral blood mesenchymal stem cell (MSC) into nerve cell. The research was conducted in Structure and Develpoment A Laboratory of Biology Department of FMIPA from January to August 2021. The study used Completely Randomized Deasign (RAL) with 7 treatments 3 replications. The treatments was of addition of patchouli extract in the form P0 (positive control with STEMdiff Forebrain Neuron Differentiation Kit medium, P1 (Negative control with Dulbecco’s modified eagle’s medium (DMEM) without patchouli extract addition, P2 (DMEM + 3 mg/ml patchouli extract), P3 (DMEM + 5 mg/ml patchouli extract), P4 (DMEM + 7 mg/ml patchouli extract), P5 (DMEM + 9 mg/ml patchouli extract), and P6 (DMEM + 11 mg/ml patchouli extract). The parameters measured were the number of proliferation which is calculated using Population Doubling Time (PDT) and transdifferentiation nerve cells which is calculated using cell count percentage. The results showed that patchouli extract give the same effect on each treatment tested. The fastest Population Doubling Time number be attained by P0 (223.2 hours) and P4 (321.6 hours), also had significant effect on induction of the number of nerve cell differentiation (P