Electronic Theses and Dissertation

Penelitian tentang isolasi, karakterisasi, dan uji bioaktif metabolit sekunder spons neopetrosia sp. pada sel kanker kolon telah dilakukan dengan pengambilan sampel pada agustus 2019 dan digunakan untuk penelitian ini pada juni hingga juli 2021. penelitian ini bertujuan untuk menemukan bukti ilmiah tentang bioaktif (ic50) metabolit sekunder ekstrak spons neopetrosia sp. terhadap sel kanker hct116 dan mengetahui struktur molekul dari metabolit sekundernya. identifikasi taksonomi sampel dilakukan di naturalis biodiversity center, the netherland dengan kode sampel s2a001. proses isolasi dan karakterisasi struktur molekul dilakukan di laboratorium kimia laut dan bioteknologi perikanan, fakultas kelautan dan perikanan, universitas syiah kuala. uji bioaktif pada lini sel kanker kolon (hct116) dan pengambilan data spektroskopi ftir hresims, nmr dilakukan di marine natural product chemistry laboratory, chemistry, biology and marine science department, faculty of science, university of the ryukyus. isolasi dilakukan dengan metode ekstraksi secara maserasi. proses partisi dilakukan secara cair-cair yang menghasilkan fraksi n-heksana, fraksi kloroform, fraksi etil asetat dan fraksi metanol. fraksi kloroform dilakukan fraksinasi dengan metode kromatografi lapis tipis preparatif menggunakan eluen kloroform : n-heksana (9:1) diperoleh bercak bulat dengan nilai rf: 0,57 dan dikoleksi dengan kode b57. uji bioaktif terhadap sel kanker hct116 dengan metode asai mtt pada b57 menunjukkan aktif dalam menghambat aktivitas sel kanker hct116 dengan nilai ic50 sebesar 10,79 μg/ml. karakterisasi spektra ftir terhadap b57 menunjukkan adanya gugus fungsi o-h (3418 cm-1) dan c-h (2929 cm-1 dan 2861 cm-1). karakterisasi spektra hresims terhadap b57 menunjukkan rumus molekul yaitu c27h46o dengan berat molekul 387,36 g/mol. karakterisasi spektra nmr 1d dan 2d menunjukkan b57 merupakan senyawa dari golongan sterol dengan nama 3s-ent-kolesten-3-ol. kata kunci: isolasi, karakterisasi, sel hct116, ic50, neopetrosia sp.  

Research on the isolation, characterization, and bioactive test of secondary metabolites of sponge Neopetrosia sp. on colon cancer cells has been carried out by sampling in August 2019 and used for this study from June to July 2021. This study aimed to find scientific evidence of bioactive (IC50) secondary metabolites of Neopetrosia sp. against HCT116 cancer cells and determine the molecular structure of its secondary metabolites. The taxonomic identification of the sample was carried out at the Naturalis Biodiversity Center, The Netherland with the sample code S2A001. The process of isolation and characterization of the molecular structure were carried out at the Laboratory of Marine Chemistry and Fisheries Biotechnology, Faculty of Marine Affairs and Fisheries, Syiah Kuala University. Bioactive test on colon cancer cell line (HCT116) and FTIR HRESIMS, NMR spectroscopic data collection were carried out at the Marine Natural Product Chemistry Laboratory, Chemistry, Biology and Marine Science Department, Faculty of Science, University of The Ryukyus. Isolation was carried out by maceration extraction method. The partition process was carried out in a liquid-liquid manner which resulted in the n-hexane fraction, chloroform fraction, ethyl acetate fraction and methanol fraction. The chloroform fraction was fractionated using a preparative thin layer chromatography method using chloroform eluent: n-hexane (9:1) to obtain round spots with an Rf value of 0.57 and were collected with the code B57. The bioactive test on HCT116 cancer cells using the MTT assay method at B57 showed that it was active in inhibiting the activity of HCT116 cancer cells with an IC50 value of 10.79 μg/mL. FTIR spectra characterization of B57 showed the presence of functional groups O-H (3418 cm-1) and C-H (2929 cm-1 and 2861 cm-1). The HRESIMS spectra characterization of B57 showed that the molecular formula was C27H46O with a molecular weight of 387.36 g/mol. Characterization of 1D and 2D NMR spectra showed that B57 was a compound from the sterol group with the name 3S-ent-cholesten-3-ol. Keywords: Isolation, characterization, HCT116 cells, IC50, Neopetrosia sp.