Electronic Theses and Dissertation

Abstrak yoghurt adalah salah satu produk fermentasi susu dengan bantuan bakteri asam laktat (bal), yoghurt memiliki manfaat sebagai pencegahan diare disebabkan eschericia coli. yoghurt dapat menjadi alternative lain pengobatan diare disebabkan bakteri selain penggunaan antibiotik. penelitian betujuan untuk mengetahui efek pemberian yoghurt yang ditambahkan filtrat pandan wangi terhadap aktivitas fagositosis makrofag mencit yang telah di infeksi escherichia coli. penelitian ini menggunakan rancangan acak lengkap (ral). hewan coba yang digunakan mencit (mus musculum) jantan umur 3-4 bulan, berat 20-30 g. perlakuan penelitian dibagi menjadi 4 perlakuan, yaitu p1 sebagai kontrol negatif diberikan aquades, p2 sebagai kontrol positif diberikan eschericia coli, p3 yoghurt pandan wangi 17,5% dan eschericia coli, p4 yoghurt pandan wangi 22,5% dan eschericia coli. penelitian ini dilakukan selama sembilan hari,pemberian perlakuan dilakukan sampai hari ketujuh,penginfeksian dilakukan pada hari kedelapan, pengambilan sampel dilakukan pada hari kesembilan. penghitungan makrofag dilakukan dengan membuat preparat apus, kemudian dilakukan pengamatan dibawah mikroskop. data hasil penelitian dianalisis menggunakan analysis of varians dan dilanjutkan dengan uji duncan. berdasarkan hasil penelitian disimpulkan pemberian yoghurt pandan wangi dapat meningkatkan aktivitas fagositosis makrofag. kata kunci : yoghurt pandan wangi, diare, escherichia coli , makrofag, mencit abstract yoghurt is a fermented milk product with lactic acid bacteria (lab), yoghurt has benefits as a prevention of diarrhea caused by eschericia coli. yoghurt can be an alternative treatment for diarrhea caused by bacteria in addition to the use of antibiotics. the purpose of this study was to determine the effect of giving pandan wangi yoghurt added to the phagocytic activity of macrophages in mice that had been infected with escherchia coli. this study used a completely randomized design (crd). the experimental animals used were male mice (mus musculus) ; 3-4 months old ; 20-30 g weight. the research treatment was divided into 4 treatments, p1 is a negative control (given aquades) ; p2 is a positive control (given eschericia coli) ; p3 pandan wangi yoghurt 17,5% with eschericia coli ; p4 pandan wangi yoghurt 22,5% with eschericia coli. this study was conducted for nine days. pandan wangi yoghurt is giving from first day until seventh days. then, eschericia coli on the eight day, and sampling was carried out on the ninth day. macrophages were counted by making smear preparations, then observed under a microscope. the research data were analyzed using analysis of variance and continued with duncan's test. based on the results of the study, it was concluded that the administration of pandan wangi yoghurt could increase the phagocytic activity of macrophages. keywords : yoghurt pandan wangi filtrate, diarrhea, escherichia coli, makrophage, mice