Electronic Theses and Dissertation

Abstrak uji aktivitas antibakteri ekstrak metanol daun gaharu (aquilaria malaccensis) terhadap pertumbuhan bakteri staphylococcus aureus dan escherichia coli telah dilakukan untuk mengetahui aktivitas ekstrak metanol daun gaharu dalam menghambat pertumbuhan bakteri tersebut. ekstrak dari sampel diperoleh melalui metode maserasi dengan pelarut metanol. simplisia daun gaharu mengandung kadar air sebesar 7,83%, kadar abu total 5,64%, kadar sari larut air 17,5%, dan kadar sari larut etanol 29,17%. ekstrak metanol daun gaharu mengandung kadar air sebesar 12,83%, kadar abu total 4,77%, kadar sari larut air 28,33%, dan kadar sari larut etanol 44,17%. hasil uji fitokimia menunjukkan bahwa ekstrak mengandung senyawa alkaloid, saponin, tanin, steroid, dan glikosida. uji aktivitas antibakteri ekstrak metanol sampel dilakukan dengan menggunakan metode sumuran dengan variasi konsentrasi ekstrak yaitu 5; 10; 15; 20 dan 25%. aktivitas antibakteri terhadap s. aureus pada ekstrak metanol daun gaharu lebih tinggi dibandingkan dengan aktivitas ekstrak metanol pada bakteri e. coli. aktivitas tertinggi ekstrak metanol daun gaharu terhadap s. aureus dan e. coli diperoleh pada konsentrasi 25% dengan zona hambat masing-masing 12 mm dan 7,8 mm. kata kunci: daun gaharu, aquilaria malaccensis, ekstrak metanol, antibakteri abstract antibacterial activity test of methanolic extract from agarwood leaves (aquilaria malaccensis) towards bacterial growth of staphylococcus aureus and escherichia coli has been conducted to know the activity of metanolic extract from agarwood leaves in inhibiting the growth of those bacterials. the extract of the samples were obtained through maceration method by methanol as a solvent. agarwood leaves simplicial characterization contains water content of 7,83%, total ash content of 5,64%, water soluble extract content of 17,5%, and ethanol soluble extract content of 29,17%. methanolic extract from agarwood leaves contains water content of 2812,83%, total ash content of 4,77%, water soluble extract content of 28,33%, and ethanol soluble extract content of 44,17%. phytochemical test showed that both of extract contained alkaloid compounds, saponins, tannins, steroids, and glycosides. antibacterial activity test of methanolic extract from samples was conducted using a well diffusion method with variation of extract concentrations of 5; 10; 15; 20 and 25%. antibacterial activity towards bacterial of s. aureus at methanolic extract from agarwood leaves is higher than activity of methanol extract towards bacterial of e. coli. the highest activity of methanolic extract from agarwood leaves towards s. aureus and e.coli were obtained at 25% concentration with inhibiting zone diameter of 12 mm and 7,8 mm. keywords: agarwood leaves, aquilaria malaccensis, methanolic extract, antibacterial.