Electronic Theses and Dissertation

Abstrak resistensi antibiotik terhadap bakteri mendorong dilakukannya berbagai penelitian untuk mendapatkan bahan baku obat yang berasal dari alam sebagai antibakteri. penelitian ini menguji aktivitas antibakteri ekstrak n-heksana, etil asetat, dan metanol daun peria laut (colubrina asiatica (l.) brongn) terhadap bakteri methicillin resistants staphylococcus aureus (mrsa). ekstraksi daun peria laut dilakukan dengan metode maserasi bertingkat menggunakan ekstrak n-heksana, etil asetat, dan metanol. terhadap masing-masing ekstrak dilakukan uji fitokimia dan karakterisasi ekstrak. uji aktivitas antibakteri dilakukan menggunakan metode kirby-bauer dengan variasi konsentrasi 5, 10, 15, dan 20%. hasil uji fitokimia menunjukkan bahwa ekstrak n-heksana tidak memberikan hasil positif terhadap golongan senyawa alkaloid, saponin, tanin, flavonoid, dan steroid/triterpenoid. ekstrak etil asetat mengandung senyawa saponin dan steroid. ekstrak metanol mengandung senyawa tanin, flavonoid, saponin dan steroid. hasil karakteristik ekstrak n-heksana, etil asetat dan metanol menunjukkan kadar air sebesar 6,70; 32,11; dan 16,52%, kadar sari larut air sebesar 4,31; 2,65; dan 16,52%, kadar sari larut etanol sebesar 8,28; 56; dan 54,52%, kadar abu total sebesar 0,14; 6,37; dan 4,83%. hasil uji aktivitas antibakteri menunjukkan bahwa ekstrak n-heksana dan metanol tidak memiliki zona hambat, sedangkan ekstrak etil asetat menunjukkan zona hambat terbesar pada konsentrasi 20% dengan ratarata diameter zona hambat 10,49 mm. kata kunci: colubrina asiatica (l.) brongn, methicillin resistants staphylococcus aureus, dan metode kirby-bauer. abstract antibiotic resistence on bacterial encourages varicus research to find raw material drugs from nature as antibacterial. antibacterial activity test of n-hexane, ethyl acetate, and methanol extract of peria laut leaves was conducted againts metichillin resistant staphylococcus aureus (mrsa). extraction was accomplished using solvent multistep maceration method by n-hexana, ethyl acetate, and methanol. the charactetization of extract and phytochemical test were evaluated for peria laut leaves extract. antibacterial activity was determined by kirby-bauer method with variation of concentration 5, 10, 15, and 20%. phytochemical test results showed that n-hexane extract was not give positive result on alkaloid, saponin, tannin, flavonoid and steroid/triterpenoid. ethyl acetate extract contains saponin and steroid compound. methanol extract contains tannin, flavonoid, and steroid. characterization extract n-hexane, ethyl acetate, and methanol extract showed that water content 6.70; 32.11; and 16.52%, water soluble extract were 4.31; 2.65; and 54.52%, ethanol soluble extract 8.28; 56.26; and 54.53%, and total ash value 0.14; 6.37; and 4.83%. the results of antibacterial activity test showed that n-hexane and methanol extract were not present inhibition zone, however ethyl acetate extract showed that the largest inhibition zone on 20% concentration with average diameter was 10,49 mm. keywords: colubrina asiatica (l.) brongn, metichillin resistant staphylococcus aureus, and kirby-bauer method.