Electronic Theses and Dissertation

Bstrak deteksi antibodi kambing terhadap virus penyakit mulut dan kuku secara serologis menggunakan metode enzyme-linked immunosorbent assay (elisa) penyakit mulut dan kuku (pmk) merupakan penyakit infeksius yang menyerang hewan berkuku belah, termasuk kambing, dan dapat menyebabkan kerugian ekonomi yang signifikan. penelitian ini bertujuan untuk mendeteksi keberadaan antibodi terhadap virus pmk pada kambing secara serologis menggunakan metode enzyme-linked immunosorbent assay (elisa). penelitian ini menggunakan 10 sampel serum kambing yang diambil secara acak dari kota banda aceh dan kabupaten aceh besar. sampel diuji menggunakan kit elisa tipe priocheck fmdv ns dengan metode inkubasi serum sehari. hasil menunjukkan bahwa dari 10 sampel yang diuji, 8 sampel menunjukkan hasil negatif, 1 sampel weak positif, dan 1 sampel positif. temuan ini mengindikasikan bahwa sudah adanya paparan virus pmk di wilayah tersebut. uji serologis menggunakan elisa dapat mendeteksi adanya antibodi terhadap virus pmk, dan dapat dijadikan sebagai metode monitoring untuk mencegah penyebaran penyakit pada populasi kambing. kata kunci : kambing, virus pmk, elisa

ABSTRACT DETECTION OF GOAT ANTIBODY TO FOOTH AND MOUTH DISEASE VIRUS SEROLOGICALLY USING Enzyme-Linked Immunosorbent Assay METHOD (ELISA) Foot-and-Mouth Disease (FMD) is an infectious disease that affects cloven hoofed animals, including goats, and can result in significant economic losses. This study aimed to detect the presence of antibodies against the FMD virus in goats through a serological approach using the Enzyme-Linked Immunosorbent Assay (ELISA) method. A total of 10 goat serum samples were randomly collected from Banda Aceh City and Aceh Besar Regency. The samples were tested using the prioCHECK FMDV NS ELISA kit with a one-day serum incubation protocol. The results showed that out of the 10 samples tested, 8 were negative, 1 was weakly positive, and 1 was positive. These findings indicate previous exposure to the FMD virus in the area. Serological testing using ELISA is capable of detecting the presence of antibodies against FMD virus and can serve as a useful tool for monitoring and preventing the spread of the disease within goat populations. Keywords : Goat, FMD Virus, ELISA.