Electronic Theses and Dissertation

Daun ketapang (terminalia catappa l.) diketahui memiliki kandungan senyawa bioaktif yang berpotensi sebagai antibakteri. penelitian ini dilakukan untuk mengetahui profil senyawa kimia ekstrak etil asetat daun t. catappa dari aceh selatan dan aceh besar serta aktivitas antibakterinya terhadap streptococcus mutans. ekstraksi daun t. catappa dilakukan dengan metode maserasi menggunakan pelarut etil asetat. simplisia daun t. catappa dikarakterisasi melalui penetapan kadar air, kadar sari larut air, kadar sari larut etanol, dan kadar abu total. analisis senyawa dilakukan menggunakan gc-ms, sedangkan aktivitas antibakteri diuji dengan metode difusi sumuran pada konsentrasi 2,5%, 5%, 10%, 15%, dan 20%. hasil karakterisasi simplisia daun t. catappa asal aceh selatan menunjukkan kadar air sebesar 1,83%, kadar sari larut air 27,5%, kadar sari larut etanol 22,5%, dan kadar abu total 6,83%. sedangkan, simplisia asal aceh besar memiliki kadar air 2,5%, kadar sari larut air 20,8%, kadar sari larut etanol 19,16%, dan kadar abu total 5,66%. hasil gc-ms menunjukkan bahwa ekstrak aceh selatan (dataran rendah) mengandung 30 senyawa, sedangkan aceh besar (dataran tinggi) mengandung 24 senyawa. senyawa yang diduga paling berperan sebagai antibakteri pada kedua ekstrak adalah 6-octadecenoic acid, n-hexadecanoic acid, phytol, squalene, dan lupeol, dengan perbedaan kadar antar lokasi, di mana aceh selatan memiliki kandungan phytol, squalene, dan lupeol yang lebih tinggi. hasil uji antibakteri menunjukkan bahwa ekstrak aceh selatan menghasilkan aktivitas lebih tinggi dengan diameter zona hambat 9,08–14,09 mm dibandingkan aceh besar 6,98–10,40 mm terhadap s. mutans. berdasarkan hasil penelitian, ekstrak etil asetat daun t. catappa dari kedua daerah memiliki aktivitas antibakteri terhadap s. mutans, dengan aktivitas yang lebih tinggi pada sampel asal aceh selatan. kata kunci: terminalia catappa, ekstrak etil asetat, gc-ms, dataran rendah dan tinggi, streptococcus mutans, antibakteri

Ketapang leaves (Terminalia catappa L.) are known to contain bioactive compounds with potential antibacterial activity. This study was conducted to determine the chemical profile of the ethyl acetate extract of T. catappa leaves collected from Aceh Selatan and Aceh Besar, as well as to evaluate their antibacterial activity against Streptococcus mutans. Extraction of T. catappa leaves was carried out by maceration using ethyl acetate as the solvent. The simplicia of T. catappa leaves were characterized through the determination of moisture content, water-soluble extractive value, ethanol-soluble extractive value, and total ash content. Compound analysis was carried out using GC-MS, while antibacterial activity was tested using the agar well diffusion method at concentrations of 2.5%, 5%, 10%, 15%, and 20%. The results of simplicia characterization of T. catappa leaves from Aceh Selatan showed a moisture content of 1.83%, water-soluble extract content of 27.5%, ethanol-soluble extract content of 22.5%, and total ash content of 6.83%. Meanwhile, simplicia from Aceh Besar had a moisture content of 2.5%, water soluble extract content of 20.8%, ethanol-soluble extract content of 19.16%, and total ash content of 5.66%. GC-MS analysis showed that the Aceh Selatan extract (lowland) contained 30 compounds, while the Aceh Besar extract (highland) contained 24 compounds. The compounds presumed to play the most important role as antibacterial agents in both extracts were 6-octadecenoic acid, n-hexadecanoic acid, phytol, squalene, and lupeol, with differences in their levels between locations, where Aceh Selatan had higher contents of phytol, squalene, and lupeol. The antibacterial test results showed that the Aceh Selatan extract exhibited higher activity with inhibition zone diameters of 9.08–14.09 mm compared to Aceh Besar, which showed 6.98–10.40 mm against S. mutans. Based on the results of the study, ethyl acetate extracts of T. catappa leaves from both regions showed antibacterial activity against S. mutans, with higher activity observed in the Aceh Selatan sample. Keywords: Terminalia catappa, ethyl acetate extract, GC-MS, lowland and highland, Streptococcus mutans, antibacterial.