Electronic Theses and Dissertation

Hornstedtia conoidea ridl., merupakan tumbuhan obat dari famili zingiberaceae yang mengandung flavonoid, saponin dan terpenoid serta berpotensi sebagai agen antioksidan. diabetes melitus dapat merusak ginjal, otak dan limpa melalui stres oksidatif dan inflamasi. penelitian ini bertujuan membandingkan perubahan histopatologis ginjal, otak dan limpa tikus diabetes setelah pemberian ekstrak metanol daun hornstedtia conoidea ridl. (emdhc), serta mengevaluasi pengaruh dosis ekstrak terhadap struktur histopatologis ketiga organ. penelitian ini menggunakan metode eksperimental dengan rancangan acak lengkap (ral) yang terdiri dari 6 perlakuan dan 4 ulangan. induksi diabetes melitus terhadap tikus (rattus norvegicus l.) dengan pemberian intraperitonial streptozotocin (stz) dosis 40 mg/kgbb terhadap hewan perlakuan kontrol negatif (k-; akuades), kontrol positif (k+; glibenklamid), serta perlakuan emdhc dosis 125 mg/kgbb, 250 mg/kgbb dan 500 mg/kgbb. induksi stz tidak dilakukan pada perlakuan kontrol normal (k0). tikus diabetes kemudian diberikan akuades (k-), glibenklamid (k+) dan dosis emdhc secara oral selama 14 hari. parameter yang diamati meliputi degenerasi dan nekrosis sel tubulus proksimal dan distal ginjal serta sel purkinje serebelum secara kuantitatif, serta struktur histologis limpa secara deskriptif. hasil penelitian menunjukkan bahwa pemberian emdhc pada dosis (125, 250 dan 500 mg/kg bb) memperbaiki histopatologi ginjal, otak dan limpa tikus diabetes, ditandai dengan penurunan derajat nekrosis pada ginjal dan otak serta perbaikan distribusi limfosit pada limpa. efek perbaikan organ meningkat seiring dosis, dengan dosis 500 mg/kg bb menunjukkan perbaikan histopatologis paling optimal dibandingkan dosis 125 mg/kg bb dan 250 mg/kg bb.

Hornstedtia conoidea Ridl. is a medicinal plant belonging to the Zingiberaceae family that contains bioactive compounds such as flavonoids, saponins, and terpenoids, which have potential antioxidant properties. Diabetes mellitus can cause damage to various organs, including the kidney, brain, and spleen, through mechanisms involving oxidative stress and inflammation. This study aimed to compare histopathological changes in the kidney, brain, and spleen of diabetic rats after administration of methanolic extract of Hornstedtia conoidea Ridl. leaves (EMDHC), as well as to evaluate the effect of different extract doses on the histopathological features of these organs. This research was conducted using an experimental method with a Completely Randomized Design consisting of six treatments and four replications. Diabetes mellitus was induced in rats (Rattus norvegicus L.) by intraperitoneal injection of streptozotocin (STZ) at a dose of 40 mg/kg body weight in all groups except the normal control (K0). The treatment groups included negative control (distilled water), positive control (glibenclamide), and EMDHC at doses of 125 mg/kg BW, 250 mg/kg BW, and 500 mg/kg BW. Diabetic rats were then treated orally for 14 days. Observed parameters included degeneration and necrosis of proximal and distal renal tubular cells, as well as cerebellar Purkinje cells quantitatively, and spleen histology descriptively. The results showed that EMDHC improved histopathological conditions of the kidney, brain, and spleen, indicated by reduced necrosis and improved lymphocyte distribution. The effect increased with dose, with 500 mg/kg BW showing the most optimal improvement.