Electronic Theses and Dissertation

Penelitian ini bertujuan mengetahui, kandungan fenolik total, dan aktivitas antioksidan ekstrak etanol 96% buah pare hijau dan pare kuning (momordica charantia l.). sampel berasal dari desa paya keureuleh, aceh besar, yang dipilih menggunakan teknik purposive sampling berdasarkan kriteria jenis pare hijau dan pare kuning. simplisia dibuat melalui proses pengeringan, lalu diekstraksi dengan metode maserasi menggunakan etanol 96%. rendamen simplisia buah pare hijau sebebsar 6,61 % dan buah pare kuning sebesar 6,31%. rendemen ekstrak pare hijau sebesar 21% dan pare kuning sebesar 24,4%. penapisan fitokimia menunjukkan kedua ekstrak mengandung flavonoid, polifenol, tanin, saponin, steroid/triterpenoid. penetapan fenolik total dilakukan dengan metode spektrofotometri uv-vis menggunakan pereaksi folin-ciocalteu pada panjang gelombang 750 nm. kadar fenolik total pare hijau sebesar 20,843 ± 0,935 mg gae/g, lebih tinggi dibanding pare kuning sebesar 18,663 ± 0,252 mg gae/g. uji aktivitas antioksidan dilakukan dengan metode dpph menggunakan microplate reader pada panjang gelombang 517 nm. nilai ic50 pare hijau sebesar 75,376 ppm dan pare kuning sebesar 81,794 ppm, keduanya termasuk antioksidan kuat. nilai aai menunjukkan pare hijau sebesar 0,663 dan pare kuning sebesar 0,611, yang termasuk kategori aktivitas antioksidan sedang. vitamin c sebagai kontrol positif memiliki ic50 sebesar 2,399 ppm. hasil ini menunjukkan ekstrak buah pare memiliki aktivitas antioksidan yang baik, dengan pare hijau lebih tinggi dibandingkan pare kuning, sejalan dengan kandungan fenolik totalnya. kata kunci: momordica charantia l., fenolik total, antioksidan, dpph, ekstrak etanol

This study aims to determine the total phenolic content and antioxidant activity of 96% ethanol extracts of green and yellow bitter melon (Momordica charantia L.). Samples were obtained from Paya Keureuleh Village, Aceh Besar, using a purposive sampling technique based on the criteria of green and yellow bitter melon. The simplicia were prepared through a drying process, then extracted by maceration using 96% ethanol. The simplicia yield of green bitter melon fruit was 6.61%, whereas the simplicia yield of yellow bitter melon fruit was 6.31%. The extraction yield was 21% for green bitter melon and 24.4% for yellow bitter melon. Phytochemical screening showed that both extracts contained flavonoids, polyphenols, tannins, saponins, and steroids or triterpenoids. Total phenolic content was determined using UV-Vis spectrophotometry with Folin-Ciocalteu reagent at a wavelength of 750 nm. The total phenolic content of green bitter melon was 20.843 ± 0.935 mg GAE/g, higher than that of yellow bitter melon at 18.663 ± 0.252 mg GAE/g. Antioxidant activity was tested using the DPPH method with a microplate reader at a wavelength of 517 nm. The IC50 values were 75.376 ppm for green bitter melon and 81.794 ppm for yellow bitter melon, both classified as strong antioxidants. The AAI values were 0.663 for green bitter melon and 0.611 for yellow bitter melon, which fall into the moderate antioxidant activity category. Vitamin C as a positive control showed an IC50 value of 2.399 ppm. These results indicate that bitter melon extracts have good antioxidant activity, with green bitter melon showing higher activity than yellow bitter melon, in line with its higher total phenolic content. Keywords: Momordica charantia L., total phenolic, antioxidant, DPPH, ethanol extract