Electronic Theses and Dissertation

Perbandingan kadar kortisol pada feses sapi aceh bunting pada trimester 1, 2, dan 3 secara non invasif abstrak kortisol merupakan hormon glukokortikoid yang berperan dalam mekanisme stres fisiologis dan proses persiapan partus. selama kebuntingan, dinamika kadar kortisol dapat mencerminkan kondisi fisiologis induk dan perkembangan janin. penelitian ini bertujuan untuk mengetahui perbandingan kadar kortisol pada feses sapi aceh bunting pada trimester i, ii, dan iii dengan metode non-invasif. sebanyak 30 ekor sapi aceh betina bunting dengan umur relatif seragam (4–7 tahun) dan body condition score (bcs) 3–4 digunakan sebagai sampel, yang dibagi ke dalam tiga kelompok berdasarkan trimester kebuntingan. sampel feses dikoleksi pada pagi hari dan diekstraksi menggunakan etanol 80%, kemudian dianalisis menggunakan metode enzyme-linked immunosorbent assay (elisa). data yang diperoleh dianalisis menggunakan one-way anova dan dilanjutkan dengan uji duncan. hasil penelitian menunjukkan bahwa terdapat perbedaan kadar kortisol pada sapi aceh bunting pada trimester 1, 2, dan 3, di mana kadar kortisol meningkat pada trimester pertama dengan nilai rata-rata sebesar 25,41 ng/ml, kemudian menurun pada trimester kedua menjadi 18,75 ng/ml, dan kembali meningkat pada trimester ketiga dengan nilai rata-rata sebesar 28,62 ng/ml. kata kunci: sapi aceh, kortisol, elisa.

COMPARISON OF CORTISOL LEVELS IN THE FECES OF PREGNANT ACEH COWS IN THE FIRST, SECOND, AND THIRD TRIMESTERS NON- INVASIVELY ABSTRACK Cortisol is a glucocorticoid hormone that plays a role in physiological stress mechanisms and the process of preparing for calving. During pregnancy, cortisol level dynamics can reflect the physiological condition of the mother and fetal development. This study aims to determine the comparison of cortisol levels in the feces of pregnant aceh cows in the first, second, and third trimesters using a non-invasive method. A total of 30 pregnant Aceh cows of relatively uniform age (4–7 years) and Body Condition Score (BCS) 3–4 were used as samples, which were divided into three groups based on the trimester of pregnancy. Fecal samples were collected in the morning and extracted using 80% ethanol, then analyzed using the Enzyme-Linked Immunosorbent Assay (ELISA) method. The data obtained were analyzed using One-Way ANOVA and followed by Duncan's test. The results showed that there was a significant difference cortisol levels increasing in the first trimester there were differences in cortisol levels in pregnant aceh cows in the first, second, and third trimesters, where cortisol levels increased in the first trimester with an average value of 25.41 ng/ml, then decreased in the second trimester to 18.75 ng/ml, and increased again in the third trimester with an average value of 28.62 ng//ml. Keywords: Aceh cattle, cortisol, ELISA.