Electronic Theses and Dissertation

Penggunaan antifungi dalam jangka panjang berpotensi menimbulkan efek samping dan resistensi sehingga diperlukan alternatif antifungi berbahan alam. daun ruku-ruku (ocimum tenuiflorum l.) dilaporkan mengandung senyawa bioaktif seperti flavonoid, saponin, tanin, alkaloid, dan terpenoid yang berpotensi sebagai antifungi. penelitian ini bertujuan untuk menguji aktivitas antifungi ekstrak etanol daun ruku-ruku terhadap pertumbuhan c. albicans secara in vitro. penelitian dilakukan secara eksperimental menggunakan metode difusi cakram. ekstrak etanol daun ruku-ruku diperoleh melalui metode maserasi menggunakan etanol 96% dan diuji pada konsentrasi 5%, 10%, 20%, 40%, dan 60%. jamur uji yang digunakan adalah c. albicans atcc 10231, dengan nistatin sebagai kontrol positif dan cmc 1% sebagai kontrol negatif. parameter yang diamati adalah diameter zona inhibisi yang terbentuk setelah inkubasi pada suhu 37°c selama 24 jam. data dianalisis menggunakan uji one way anova yang dilanjutkan dengan uji duncan. hasil penelitian menunjukkan bahwa seluruh konsentrasi ekstrak etanol daun ruku-ruku mampu menghambat pertumbuhan c. albicans dengan diameter zona inhibisi 6,37 mm, 7,07 mm, 8,50 mm, 10,47 mm dan 11,87 mm dengan penghambatan paling tinggi pada konsentrasi 60%. nilai penghambatan tersebut menunjukkan lebih tinggi dibandingkan dengan kontrol positif nistatin yaitu sebesar 10,23±0,15 mm. berdasarkan hasil penelitian ini dapat disimpulkan bahwa ekstrak etanol daun ruku-ruku memiliki aktivitas dan potensi sebagai agen antifungi terhadap c. albicans. kata kunci: antifungi, candida albicans, daun ruku-ruku, ocimum tenuiflorum, zona inhibisi

Long-term use of antifungals has the potential to cause side effects and resistance, so alternative natural antifungal agents are needed. Ruku-ruku leaves (Ocimum tenuiflorum L.) are reported to contain bioactive compounds such as flavonoids, saponins, tannins, alkaloids, and terpenoids that have the potential as antifungals. This study aims to test the antifungal activity of ethanol extract of ruku-ruku leaves against the growth of C. albicans in vitro. The study was conducted experimentally using the disc diffusion method. The ethanol extract of ruku-ruku leaves was obtained through the maceration method using 96% ethanol and tested at concentrations of 5%, 10%, 20%, 40%, and 60%. The test fungus used was C. albicans ATCC 10231, with nystatin as a positive control and 1% CMC as a negative control. The observed parameter was the diameter of the inhibition zone formed after incubation at 37°C for 24 hours. Data were analyzed using the One Way ANOVA test followed by the Duncan test. The results showed that all concentrations of ethanol extract of ruku-ruku leaves were able to inhibit the growth of C. albicans with inhibition zone diameters of 6.37 mm, 7.07 mm, 8.50 mm, 10.47 mm and 11.87 mm with the highest inhibition at a concentration of 60%. The inhibition value was higher than the positive control Nystatin which was 10.23 ± 0.15 mm. Based on the results of this study, it can be concluded that the ethanol extract of ruku-ruku leaves has activity and potential as an antifungal agent against C. albicans. Keywords: antifungal, Candida albicans, inhibition zone, Ocimum tenuiflorum, ruku-ruku leaf