Electronic Theses and Dissertation

Karies gigi merupakan penyakit infeksi kronis yang berkaitan erat dengan pembentukan biofilm oleh streptococcus mutans. struktur biofilm meningkatkan resistensi bakteri terhadap agen antimikroba dan mekanisme pertahanan host, sehingga menyulitkan pengendalian infeksi. pengembangan agen antibiofilm menjadi penting dalam pencegahan dan pengobatan karies gigi. minyak nilam (pogostemon cablin benth.) merupakan bahan alam yang berpotensi dikembangkan sebagai agen antimikroba. penelitian ini bertujuan untuk mengetahui pengaruh antibiofilm minyak nilam fraksi berat terhadap biofilm s. mutans pada tikus rattus norvegicus yang mengalami karies. penelitian ini merupakan penelitian eksperimental laboratoris in vivo dengan desain pretest–posttest only control group. hewan coba dibagi menjadi kelompok kontrol negatif (akuades), kontrol positif (amoksisilin), serta kelompok perlakuan minyak nilam fraksi berat dan fraksi ringan. induksi karies dilakukan sebelum pemberian bahan uji, kemudian perlakuan diberikan selama enam hari. analisis data dilakukan menggunakan uji paired t-test , one way anova dan post hoc lsd. hasil penelitian menunjukkan peningkatan massa biofilm setelah manipulasi karies. kelompok kontrol negatif memiliki massa biofilm tertinggi, sedangkan kontrol positif terendah. pemberian minyak nilam menurunkan massa biofilm dibandingkan kontrol negatif dengan fraksi berat menunjukkan efek lebih kuat dibandingkan fraksi ringan, meskipun tidak berbeda bermakna secara statistik. minyak nilam fraksi berat berpotensi sebagai agen antibiofilm alami terhadap s. mutans pada kondisi karies gigi.

Dental caries is a chronic infectious disease closely associated with biofilm formation by Streptococcus mutans. The biofilm structure increases bacterial resistance to antimicrobial agents and host defense mechanisms, thereby complicating infection control. Therefore, the development of effective antibiofilm agents is essential for the prevention and treatment of dental caries. Patchouli oil (Pogostemon cablin Benth.) is a natural product with potential antimicrobial properties. This study aimed to evaluate the antibiofilm effect of the heavy fraction of patchouli oil on S. mutans biofilm in caries-induced Rattus norvegicus. This study was an in vivo laboratory experimental study using a pretest–posttest only control group design. The animals were divided into a negative control group (distilled water), a positive control group (amoxicillin), and treatment groups receiving the heavy and light fractions of patchouli oil. Caries induction was performed prior to treatment, followed by six days of intervention. Data were analyzed using the Paired T-test, One Way ANOVA, and Post Hoc LSD. The results showed an increase in biofilm mass after caries manipulation. The negative control group exhibited the highest biofilm mass, whereas the positive control group showed the lowest. Patchouli oil treatment reduced biofilm mass compared to the negative control, with the heavy fraction demonstrating a stronger antibiofilm effect than the light fraction, although the difference was not statistically significant. The heavy fraction of patchouli oil has potential as a natural antibiofilm agent against S. mutans in dental caries conditions.