Electronic Theses and Dissertation

Diet tinggi lemak dan stres meningkatkan stres oksidatif melalui pembentukan reactive oxygen species (ros) dan peroksidasi lipid yang ditandai oleh peningkatan malondialdehyde (mda), yang selanjutnya memicu degradasi matriks ekstraseluler kulit melalui peningkatan ekspresi matriks metalloproteinase-1(mmp-1). dampak tersebut dapat dicegah dengan pemberian antioksidan. kopi robusta memiliki sifat antioksidan dan antiinflamasi kuat yang berpotensi melindungi tubuh. penelitian bertujuan membuktikan pengaruh seduhan kopi robusta terhadap kadar trigliserida, mda serum dan ekspresi mmp-1. metode penelitian in vivo dengan rancangan post-test only control design menggunakan 16 ekor tikus wistar jantan. subjek dibagi ke dalam empat kelompok yaitu tikus sehat yang tidak diberi diet tinggi lemak stres dan seduhan kopi robusta (k1), tikus yang diberikan diet tinggi lemak dan stres (k2), tikus diberikan diet tinggi lemak, stres, dan seduhan kopi robusta (k3), dan tikus hanya diberi seduhan kopi robusta (k4). intervensi dilakukan selama 28 hari. kadar trigliserida diukur dengan metode chod-pap, sementara mda serum dan ekspresi mmp-1 dianalisis menggunakan elisa. hasil menunjukkan perbedaan signifikan kadar trigliserida, mda serum dan ekspresi mmp-1 pada setiap kelompok (p

High-fat diet and stress exacerbate oxidative stress via Reactive Oxygen Species generation and lipid peroxidation, characterized by elevated Malondialdehyde (MDA). This condition subsequently upregulates Matrix Metalloproteinase-1 (MMP-1), leading to skin extracellular matrix degradation. Robusta coffee, known for its potent antioxidant properties, may mitigate these deleterious effects. This study evaluated the effect of Robusta coffee infusion on triglyceride, serum MDA, and MMP-1 levels. This in vivo study used a post-test only control group design with 16 male Wistar rats divided into four groups: healthy controls (K1); HFD and stress (K2); HFD, stress, and Robusta coffee infusion (K3); and Robusta coffee infusion only (K4). After a 28-day intervention, triglycerides were measured using CHOD-PAP, while MDA and MMP-1 were analyzed via ELISA. Significant differences were observed across all groups (p < 0.05). LSD post-hoc analysis demonstrated that the treatment group (K3) exhibited significantly lower triglyceride, MDA, and MMP-1 levels compared to the HFD and stress group (K2) (p < 0.05). Similarly, the K4 group showed significantly lower levels for all parameters compared to K2 (p < 0.05).Robusta coffee infusion demonstrated protective effects by reducing triglyceride and serum MDA levels and inhibiting skin aging through suppression of MMP-1 expression in rats subjected to a high-fat diet and stress. Keywords: Robusta coffee, triglycerides, malondialdehyde, MMP-1, high-fat diet, oxidative stress.